FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_84.35100000044dbf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_84.35100000044dbf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC96.976744186046512No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT32.3255813953488373No Hit
ATGTTACCATATCAAGCTGAAAATGTCACCACTATCTGGAGATTTCGACGT21.550387596899225No Hit
GTAATGAATGATACCCAATGTGCCTTTTCTAGAAAAAGTTGCCAAATATAT10.7751937984496124No Hit
GCTTAAGGCTGCTGGTGCCACCTGGATTCAGCTTGACGAGCCTGTCCTTGT10.7751937984496124No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGCT10.7751937984496124No Hit
GTACTCTGCGTTGATACCACCCCGTACTCCTGTCTCTTATACACATCTCCG10.7751937984496124No Hit
ATATTGGAATGGTGGTAATACGAGGAAATAGTATCATCATGCTGTCTCTTA10.7751937984496124No Hit
TGCCTTATCTCGTATGCCGTCTTCCGCTTGAAAAAAAAAATCGAAACGCTA10.7751937984496124Illumina PCR Primer Index 10 (96% over 27bp)
GAGTATATCAAGGTTCATCACAAGACCTATGAAGATATAGATAAACGTGGA10.7751937984496124No Hit
TTTTTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTTATA10.7751937984496124No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACTC10.7751937984496124No Hit
GAGTACGGGGGGGAAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAGTA10.7751937984496124No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACCCGAAAAA10.7751937984496124Illumina PCR Primer Index 5 (96% over 28bp)
CCCTGGCCCTACTCATCAGGTTCAAGGTGAATTCGGCTATCCTCGTGGCTG10.7751937984496124No Hit
CTTACACATCTATCCTAGACATTTATAAGCACTCTAATGGATAACCTGTCT10.7751937984496124No Hit
GATGTGATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAAGTAAGACC10.7751937984496124Illumina Single End Adapter 1 (95% over 23bp)
TTCTTATACATCTCCTCCATCATGTCTGGAGTTACGCTGTTCTTTATGTAT10.7751937984496124No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAG10.7751937984496124No Hit
CTTATACACATCTCCGCCACGAGACTCGCTATGATCTCGTATGCCGTCTTC10.7751937984496124RNA PCR Primer, Index 22 (95% over 22bp)
AGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGT10.7751937984496124No Hit
AATGAAATGTTCAACTCTGTGAAATGAATGCACTCATCACAAAGAAGTTTC10.7751937984496124No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTCTCTTATAC10.7751937984496124RNA PCR Primer, Index 27 (96% over 28bp)
GGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGA10.7751937984496124No Hit
GTACGGGAAGCAGTGGTAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGT10.7751937984496124No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGC10.7751937984496124No Hit
CCCCACTTCTGTGTTCCTATGGTTCAAATCCGTTGAAGCTGCTAATAAGGA10.7751937984496124No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACCCGTCTCTTATACACATCT10.7751937984496124No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATACGTAGGTAAGA10.7751937984496124Illumina PCR Primer Index 8 (96% over 27bp)
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCGTATGCCGTC10.7751937984496124RNA PCR Primer, Index 36 (96% over 29bp)
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAACAAAACAAGAGATCCC10.7751937984496124Illumina PCR Primer Index 5 (96% over 28bp)
GTAGTAAAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGACTG10.7751937984496124No Hit
GTATCAACGCAGAGTACGGGTGGTATCATGCAGAGTACTTTTTTTTTTTTT10.7751937984496124No Hit
GTTAAGAAGGAGAAAAATCGTTGGTTTGGGAATCTCCATCATTTTTCAAGC10.7751937984496124No Hit
GTTATATGATGATGCTATTATACTACAAAGTGGTGGGTTTTTGTTTGGTCT10.7751937984496124No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAATAGA10.7751937984496124RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAACCATGATCTCGTATGCCGTC10.7751937984496124No Hit
CCTTTTATTTGACTTTGCATTTTTATCAACGATGGCAAAAAATCACACAAC10.7751937984496124No Hit
ATGTAACACAAAATCAAGTTCTTGAAACATAAAGTAGAACAGAACAAGACT10.7751937984496124No Hit
TTCTTTAACTTTTTGTTAGGGATTCACTCATGTTTCTGTCTCTTATACACA10.7751937984496124No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCCCCCCGTACTC10.7751937984496124No Hit
GTCTAGAAGGAAGAGGACTGAAATGAAAGAAGACCTGTCTCTTATACACAT10.7751937984496124No Hit
ATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTT10.7751937984496124No Hit
CTTTAATGCTGCAGAAAATTTCAAATCACCCTTGATAATCCACTTTCTTTT10.7751937984496124No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATATTCCTTCG10.7751937984496124RNA PCR Primer, Index 27 (96% over 28bp)
CTACAAAATGTGGTGATCATGTGATTTGATACAATTTAGTTCTTGCAGTGT10.7751937984496124No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC10.7751937984496124No Hit
CATACACAAAGACATGTAGGTACAAACTGTTCTGTAAATACCTCTGCCCAT10.7751937984496124No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10.7751937984496124No Hit
CATCTGAAGTCTTTGTTCTAACACATTCTCATAGAACTGTCTCTTATACAC10.7751937984496124No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCGGG10.7751937984496124No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGCAAGATCTCGTATGCCGTC10.7751937984496124No Hit
GAAAGACATTACATGAAAGAATGCTCATGGAAAACTCTTATGAGCAATAAC10.7751937984496124No Hit
GATATCAACTATACTGACCGAAGATTCTCCTCCATTAACCTGGAATAAGCT10.7751937984496124No Hit
CCCTTAAACAGCCCCGTTTCTGTTAGAAAAGGCTGATAGGAAATGTATTGC10.7751937984496124No Hit
ATATACAGTTGGATAACATTTTGATTACTACAAAGTTGTTCTTCCTGGCTT10.7751937984496124No Hit
AATACATATTGACTCATGTTTTTGTCCATTAATGGTCCGTGGGTTTCTTTA10.7751937984496124No Hit
GAACTAACTGTATACAGTTAGTTCCTACTCTCCAACTGAACATAATAAGCA10.7751937984496124No Hit
GTATCAACGCAGAGTACGGGTGGCTGTCTCTTATACACATCTCCGAGCCCA10.7751937984496124No Hit
GAACATGCCTTTAAAGACACGCTCGAAGGCAAGCTTAGAGGCGTTAGGGTC10.7751937984496124No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTGTGGTCAATT10.7751937984496124RNA PCR Primer, Index 36 (96% over 29bp)
CTCTTTCTTTCTTCTTCCTTTTTCTCTCTCCCTTCTTCTTTTTTCCCTGTC10.7751937984496124No Hit
GCTCGGAGATGTGTATAAGAGACAGTCATTAACCTATGGACTGTCTCTTAT10.7751937984496124No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGCTGTCTCTTATACACAT10.7751937984496124No Hit
GGATACATTAGCATGGGATAACATCATAGGATTTCGATCCTATTGTGTCTG10.7751937984496124No Hit
GTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGTGAGCAT10.7751937984496124No Hit
TGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTGTCTC10.7751937984496124No Hit
GTCACACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTG10.7751937984496124No Hit
ATCAATAAGGAGTCAAAATCCAGTGACACATTCATAAAAACTCCATATATC10.7751937984496124No Hit
ATCTTACATTAAGCATGAGTCTAATTTGTATCTGTCTCTTATACACATCTC10.7751937984496124No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCCTGATCTCGTATGCCGTC10.7751937984496124No Hit
CACTATTTCTGTTTTACAGCAGTTTTGAAACTGTCTCTTATACACATCTCC10.7751937984496124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTCA10.7751937984496124No Hit
ATGTAGAATTTTTGTAAGGGAGGTGACGATGACTTGCCCACTGTGAGAATT10.7751937984496124No Hit
AAAACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATACACA10.7751937984496124No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCAAAGAGAAA10.7751937984496124RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCAGGAAGTA10.7751937984496124RNA PCR Primer, Index 27 (96% over 28bp)
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATATGTAAATGA10.7751937984496124No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC10.7751937984496124No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAATATCCG10.7751937984496124Illumina PCR Primer Index 8 (96% over 27bp)
GGATGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAA10.7751937984496124No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTAAACCTGT10.7751937984496124Illumina PCR Primer Index 8 (96% over 27bp)
CCCAAGGCCTTAATTGCTGAAGCTCTCAATATGTATTGGTGGTAAGTAGAC10.7751937984496124No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTAGAAAAAAAAGACTCTTATACAC10.7751937984496124Illumina PCR Primer Index 10 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACGAATAA10.7751937984496124Illumina PCR Primer Index 8 (96% over 27bp)
CTGTAGACATCTCTGTGAAGAGCAAGCTATCATTAAACATGCCTGCTTATC10.7751937984496124No Hit
AAGAAGGGTTGTATGAAAGGTAAAGGAGGACCAGAGACTGTCTCTTATACA10.7751937984496124No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACCAACACGCC10.7751937984496124Illumina PCR Primer Index 8 (96% over 27bp)
AGGATATCATGCAGAGCGTCGATTGATTTGGATATCTATTTGGTATTTGCT10.7751937984496124No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATA10.7751937984496124No Hit
CTACCTGGTTCGTGCTTTTATTACTGAAGACGTTAACGCGACGTAAAGCAT10.7751937984496124No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACCT10.7751937984496124No Hit
TTTGTATAGAGCAGAGTTTTAAAATGAATGTAAATAGCACTAAACGTTTTC10.7751937984496124No Hit
GCTCGCCGAACGTCGTGCACTGGGTCTACGACACCTACATGCTGGCCAACA10.7751937984496124No Hit
TTGTAGATGTGGTTTGACTATTTCTGTATGTCTCCATCTATCTGTCTCTTA10.7751937984496124No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCTTATAAAAG10.7751937984496124RNA PCR Primer, Index 27 (96% over 28bp)
AAAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC10.7751937984496124No Hit
TATATACTCTTACCATTGGGGGTATTGTTGTATTGGTTTGTATTATGACTG10.7751937984496124No Hit
CTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACATAGTGGAAGCATTAGG10.7751937984496124Illumina Single End Adapter 1 (100% over 22bp)
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATCTACTCCTC10.7751937984496124RNA PCR Primer, Index 36 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCGGC10.7751937984496124No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGATGAAGAAG10.7751937984496124Illumina PCR Primer Index 8 (96% over 27bp)
GATGAAGGGCGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG10.7751937984496124No Hit
ATTTCTTACCCTCTGATAACTTAGGCAAATGAGCTGTCTCTTATACACATC10.7751937984496124No Hit
AAAAAGACTGGCCTGGGATGAGTATTTTGCAGCCCAAGCATTACTACTGTC10.7751937984496124No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCA10.7751937984496124No Hit
AAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTGGTTT10.7751937984496124No Hit
CATTAGCAGAAGTCATGTTCCAGCTTAGACTGATGAAGAATTAAAAATCTG10.7751937984496124No Hit
TGTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTATAATCTCC10.7751937984496124RNA PCR Primer, Index 15 (96% over 29bp)
ACCTAAATAGCTTTCGGGGAGAACCAGCTATCTCCCGGTTTGATTGGCCTT10.7751937984496124No Hit
ATACTAGTGAGACCCCATCCCTACAAAAAAAATTAAAATTAGCCAAGTGCA10.7751937984496124No Hit
GTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTG10.7751937984496124No Hit
GTATCAACGCAGAGTACGGGAGTGGTATAAACGCAAAAAAAAAAAAAAAAA10.7751937984496124No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCAACACCCAC10.7751937984496124Illumina PCR Primer Index 5 (96% over 28bp)
GTGGCCAGGTCCCTGTTCCGGTCCTCCTCCGACCGGCTGGTGTTCCTGAGC10.7751937984496124No Hit
GGTGAAAGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGT10.7751937984496124No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAATCCC10.7751937984496124Illumina PCR Primer Index 5 (96% over 28bp)
GGCCCATGTCCTCAGGCAACATAAGATAGCGCATTCCTTGGCTGGTCCTTG10.7751937984496124No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers