FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_83.35100000044d96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_83.35100000044d96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC75.982905982905983No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT32.564102564102564No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC21.7094017094017095No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGT21.7094017094017095No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAACAAAAAGGCCCGAGATG10.8547008547008548RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTGCGAGCCCACGAGACCTAACTGGATCTCGTATGCCGTC10.8547008547008548No Hit
ACTCAGATCTCTGAACCAGAGTCGTTTGTGGCCAAGCACTGTCTCTTATAC10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGGCGGA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
GTATACAGAAACACAAGAAAATGAAAAGTCCCTCCGTTAGGACTGTCTCTT10.8547008547008548No Hit
CTGGAAAGGCGTGCGATACAAGGTGACAGCCCCGTACACAAAAATGCACAT10.8547008547008548No Hit
GTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTA10.8547008547008548No Hit
TTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACTATCATTAACCGAAT10.8547008547008548No Hit
CCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACC10.8547008547008548No Hit
CTAAGGGAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCCTGTC10.8547008547008548No Hit
TTAGTGTACCAAGTTAGATTCCAGAAGGTAACTTCAAATCACATGTAAAAG10.8547008547008548No Hit
CTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAG10.8547008547008548No Hit
GACCAGAGGCTGTTCACCTTGGAGACCTGATTCGGTTATGAGTACGACCGG10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGCCAGCAA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACGACGCAGG10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
TTAGATGCTAGATTCTTGACCAATTTTGTGAGATTCATACTCATAAAGACT10.8547008547008548No Hit
ACGGGGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAG10.8547008547008548No Hit
TGCTTGAACAATACGGTTGTCATCTGTAATAACGCCATCTTTTAACTGTCT10.8547008547008548No Hit
AGAAATCTTTATAAAGAACACTCAAAGGTTAGGAAAAACTCTTAATACATG10.8547008547008548No Hit
CTTTTTTGCTTTTCTTTCCCTTTTTCATTTCAACGTTCTCTTTTTTAGCCT10.8547008547008548No Hit
ATAAGACGCCAAGCGAAGACGAGAAGTCAAAAACAGTTTCCAAAATTCCTG10.8547008547008548No Hit
TAAGTACCCCGAGGCAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAG10.8547008547008548No Hit
GTGGTGGAGCATTTGATAGCAACGATGAGGAACACCAAAGGGAAGAGACCG10.8547008547008548No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAAAAAG10.8547008547008548RNA PCR Primer, Index 36 (96% over 29bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC10.8547008547008548No Hit
GTATATGACCTTTATTACCGAAGGGAAGAAATATCTAAAGAGCCTGTCTCT10.8547008547008548No Hit
GTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCCTGTCC10.8547008547008548No Hit
GTTGAAAGGACAATAAAAGAATACTATGAGCAACTGTATACTCACCTGTCT10.8547008547008548No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10.8547008547008548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGAG10.8547008547008548No Hit
CAAGAAGACTTTCATCATCATCTTCTGCCACAGAAATCTGGGATATATGTT10.8547008547008548No Hit
TCCTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTACGCCGT10.8547008547008548No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTATGCCAGC10.8547008547008548RNA PCR Primer, Index 27 (96% over 28bp)
ATAACATCCAATGACAAATCCCATAGGGACAAGAACATCTGTCTCTTATAC10.8547008547008548No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGG10.8547008547008548No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC10.8547008547008548No Hit
CCTTTATCCAACTGATGATGGAGCTCTTTATCCTTGATTCCATGCATCTAA10.8547008547008548No Hit
GTTTAAGGTTGAAGGCAAGGAAGAGAAAGAAGAAGGGGAAGATGATGAAGA10.8547008547008548No Hit
CCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCTGTCTCTTATACA10.8547008547008548No Hit
GATCTACACCGACGGCCGCTCCACCCGGGCTCGCCCCCTAGGTCTGTCTCT10.8547008547008548No Hit
GTTTAATCTTATGATCTGCTGTTTTCTTCATTAATGAGTTTCGAGTTATGG10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACAACCAAA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
AAGTTTGATTGATGTCAGTTTAGAGATACCCGAAGGTAGACTTATGTTCCT10.8547008547008548No Hit
TATTTATGCTGTCTGAAGGTCACGATCATGTTACCATATCAAGCTGAAAAT10.8547008547008548No Hit
TCTTGAATCTCTCCATACACAGGCAAAAATAAGTGTGTTACTTAACATACT10.8547008547008548No Hit
CATCCCCACCTTCCTCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCTG10.8547008547008548No Hit
ATCCAATACACGAAACAACAAACATACTATGCTGTCGCTAAGAACATTGAA10.8547008547008548No Hit
TCGTATTTCTTAAGAATGGTATTCTTTGTGCTAAGGTAAAGTAGCCACTTT10.8547008547008548No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT10.8547008547008548No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAACTGTCTCTTATACACA10.8547008547008548No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCATGAAAAAAAAAAACCCCTAGTCA10.8547008547008548Illumina PCR Primer Index 10 (96% over 27bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGACCAAGACA10.8547008547008548RNA PCR Primer, Index 27 (96% over 28bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT10.8547008547008548No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGCATTATCTCGTATGCCGTC10.8547008547008548No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGCAGCGAAAGCG10.8547008547008548RNA PCR Primer, Index 36 (96% over 29bp)
CTCTTGTAGTTTTTTATCTTTTGAGCTTAATGCCTCAAATTTTCTGTTTTC10.8547008547008548No Hit
CATACGTGCTACCCAGAGATTAATTTAGTCCATATGAACTATTGACCCATT10.8547008547008548No Hit
ATATACTCCTCCATAGTTTCTCCTCTTGTCATCTTCTGTTACGGATTCCTG10.8547008547008548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTCTGTCTCTTATACTCATCTCT10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACATCTGCCTA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
GACAGGGAACTAGGTAAGAGTAAAGTCTGATAACATGGAAAATGTTAGTGC10.8547008547008548No Hit
TCACAATTTCGAACATGATAAACAATCTTCAATGACAGATGATACTGTCTC10.8547008547008548No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTCTGTCTCTTAT10.8547008547008548No Hit
GTTTTGTACAAGTTGCTCAAGATTGTACACCTTATTCAAATAGAATCCCGT10.8547008547008548No Hit
TGACAATTTTGACTTGTTTGAAGCTGAAACAAAGTTCAATAACGCTATTAC10.8547008547008548No Hit
TCTTTGATAATCTTAACGTACTTAAAGTTTCTCATACCGAAACTCCTCTTC10.8547008547008548No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACT10.8547008547008548No Hit
GCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGA10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGCGCCCACGA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
GAAGAATGGAGAGAGAATTGAAAAAGTGGAGCATTCAGACTTGTCTTTCAG10.8547008547008548No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAAA10.8547008547008548Illumina PCR Primer Index 5 (96% over 28bp)
GAAGGGCCCCATCCAGGGACTGACTGTGGAGGAGGTAAAAAGGTCACTGGG10.8547008547008548No Hit
ATTAGAGCTGGCTTCATCCCACTTGGACTTCTGTCTCTTATACACATCTCC10.8547008547008548No Hit
GCCTTTGATAATGATGTCAGTGTAGTACTCAGGGTCGTCCTGTTGCAAAGC10.8547008547008548No Hit
GTTTGTGGCAATTCTATTGAGGAGGAAACAGGATAGTATGTACCTGTCTCT10.8547008547008548No Hit
CTTATACACATCTCCGAGCCCACAGACGTCTTAGTATCTCGTATGCCGTCT10.8547008547008548No Hit
GGACAACAGAACAATATTGGAATGGTGGTAATACGAGGAAATAGTACTGTC10.8547008547008548No Hit
CACAGTATGTACTTTTTTGTGTGTTTGTCTTTTTTCTGTCAGCATGATGGG10.8547008547008548No Hit
AAAAAGTACTCTGCGTCCCGTACTCTGCGTTGATCCCTGTCTCTTATACAC10.8547008547008548No Hit
TTGTTGCAACAGAATTTGTTGATGAAGATTTTGTTGATCTTGCTGTTGTTG10.8547008547008548No Hit
ATCACAGAATGATTATAGCAAAGGAATACATAAGTCATTCAATTTGCTTCT10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACATATATTAC10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
TTTCTGCACTAAGAGATTCTCTAGCATCCTTTATGCTTTTTTCAAGCCCAA10.8547008547008548No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA10.8547008547008548No Hit
GCTGTATCCTCAGTTGACTCATTCACTCAGTTCACACAAAAGATGTTGGAC10.8547008547008548No Hit
ATCAAACGCAGAGTACGGGTCAACGCAGAGTCTGTCTCTTATACACATCTC10.8547008547008548No Hit
GCGTTGATACCACTGCTTCCCGTACTCTTCGTTGATACCTGTCTCTTATAC10.8547008547008548No Hit
ATCTAGGCCAGAATTGACAATGTTTAAGTAATGGTGGAATCTGTCAATAAG10.8547008547008548No Hit
ATCAAGCAGAGTACGGGCCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGG10.8547008547008548No Hit
GTACCAGTCAAGGTTGGTTGACCTCTCAATCATGTTGTCTCCCTCGAATCC10.8547008547008548No Hit
GGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGTCTC10.8547008547008548No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCCTGTCTCTTATACAC10.8547008547008548No Hit
CCGTACCAAACGTCGTCGTTTCGAGGAATGGGTTGCTATGCCGCAGCAGCA10.8547008547008548No Hit
ACTCTGGACGTTTATTCCACATATCAGTTTCCCTTATTAATCCATACTTGC10.8547008547008548No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTCTCCCCATTCGGAAATCGCC10.8547008547008548No Hit
GGAAATTTTTTGTTATGATGTCTGTGTGGAAACTGTCTCTTATACACATCT10.8547008547008548No Hit
ATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACC10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAGTTTTCTAA10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)
AAGTTAGGTTCTACCTTGTTGTTTCATTAGAGCTTGTTTTAGTTGCTGTCC10.8547008547008548No Hit
GTACGGGAATTCATCCTTTCGTCTTTTTCTGTCTCTTATACACATCTCCGA10.8547008547008548No Hit
ATACTCAGTAGTGGACATGTCTTGAGATTTGTTTGGAACATGAAAGTTTTG10.8547008547008548No Hit
CTCTTGAAGTTGAGGCATCTGACTCTATTGACAACGTCTGTCTCTTATACA10.8547008547008548No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTCAGTATCG10.8547008547008548Illumina PCR Primer Index 8 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers