FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_82.35100000044d7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_82.35100000044d7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT32.7522935779816518No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC32.7522935779816518No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT21.834862385321101No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC21.834862385321101No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC21.834862385321101No Hit
ATCATGAACCACCCTGGTCAGATTGGTAACGGTTACGCCCCAGTTCTCGAT10.9174311926605505No Hit
TCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG10.9174311926605505No Hit
GTACTATCGCTAGAACAGAGCCATAAAACATAAAAACTAATAGGGTCTAGA10.9174311926605505No Hit
CATTATGAGAATTCCTAAAATGGGACAAGTGTTGTGCTCAGACTGTCTCTT10.9174311926605505No Hit
GTACGGGGGATGACTTGTGGCTGGGGGTGAAAGGCCAATCAAACCGGGAGA10.9174311926605505No Hit
TGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTC10.9174311926605505No Hit
AAATGTGTATTTACATTGTTATCTCTTGTTTCTGTCTCTTATACACATCTC10.9174311926605505No Hit
GAATATCAGTGGGGTCAGAGGTTAGCTTAGCTGCTTGCTGGGCTAGAACAG10.9174311926605505No Hit
AGCTTAAACAAAAGAGTCACAAAAAGATTCAGAATACTGATTGTGTCCAAC10.9174311926605505No Hit
ACCATATGGAATATCTGCTACAGCAGTCTTCCAAGCTGTCTCTTATACACA10.9174311926605505No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCTG10.9174311926605505No Hit
GAATATGCGGCATTTGTTTTTTTGTTCTTGCGATAGTTTACTGAGAATGAT10.9174311926605505No Hit
TTACTTTGTGGGTGATTATTTTTTTGGATTTTTTTTCATTTTTATGTATGG10.9174311926605505No Hit
GAGTACGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATAACATCTCCGAGCCCACG10.9174311926605505No Hit
ATATATGTTCTCTAGGCCTTTTAGAAAACATGGAGTTGTTCCTTTGGCCAC10.9174311926605505No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCTTGATGCT10.9174311926605505Illumina PCR Primer Index 8 (96% over 27bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10.9174311926605505No Hit
ATAATACATATCTCGAAATTCTTCCAAACACCCAAAAAAGTTGACAAAAGG10.9174311926605505No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTCTACCGT10.9174311926605505Illumina PCR Primer Index 8 (96% over 27bp)
AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC10.9174311926605505No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCACGCCT10.9174311926605505No Hit
GGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCT10.9174311926605505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT10.9174311926605505No Hit
AGTTTATTGACATCACTAAAGTTACCACTCCAGATCAGTTTGATCAACTTC10.9174311926605505No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAGAGTACTTTTTTTTTT10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCCATGATCTCGTATGCCGTC10.9174311926605505No Hit
CCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGA10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACTCACCATGATCTCGTATGCCGTC10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAACATGATCTCGTATGCCGTC10.9174311926605505No Hit
CAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTC10.9174311926605505No Hit
GTTTCAAGACGGGTCGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGC10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACCATGATCTCGTATGCCGTC10.9174311926605505No Hit
GACCATTGGATTAAATACATTACCACTGAGAATTGCAAAGGTTCAGGACAT10.9174311926605505No Hit
ACTGCTGTTTCAAAAGGAATCTTCAACTCTGTGAGTTGAATGCAATCATCA10.9174311926605505No Hit
TACCTGGAGTGTGTATTTCATCACTTTTAATGCTGTCTCTTATACACATCT10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCATGATCTCGTATGCCGTC10.9174311926605505No Hit
CTCCATAACGTTCCCTGGATAGTATTTGTCATTTCTCTTAAGTAAATGCCT10.9174311926605505No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGG10.9174311926605505No Hit
TTTACTAACTTCTCAGGTCTGTAGAACATTTCATCTTTGGTTTGAGACACA10.9174311926605505No Hit
TATCAACGCAGAGTACGGGAAGGTATCAACGCAGAGTACTTTTTCTGTCTC10.9174311926605505No Hit
CTATTCCTCCTTTCCAATTCCCTCTATCAGAGGTATTTACTGTTAATCTGG10.9174311926605505No Hit
CAGTCAATCTACGCAGAGTGAATCGGTCCCTAAGGAACCCCCGAAAGGGCT10.9174311926605505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9174311926605505No Hit
GTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCAC10.9174311926605505No Hit
ATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTCGGCATCAGCGTGCTC10.9174311926605505No Hit
GGAACTGAGTATAAGTATATTTCATCAGGGGAGGGGCTAGGACTCCCACTT10.9174311926605505No Hit
ACTAAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTATCAACGCAG10.9174311926605505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCA10.9174311926605505No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCCTGTCTCT10.9174311926605505No Hit
GGTCCAGACTCCTACGGGAGGCAGCAGTGAGGAATATTGCACAATGGGCGC10.9174311926605505No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATCATGATCTCGTATGCCGTC10.9174311926605505TruSeq Adapter, Index 1 (95% over 21bp)
GACTCGTAGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACGACGGGGCT10.9174311926605505No Hit
GTACGGGCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAG10.9174311926605505No Hit
ATATAAAATAGCAGCTCCCAGTAACTTCTTCAGGTTTTATCTTCAGAAGTT10.9174311926605505No Hit
GTATCAACGCAGAGTACGGGGAGGGGAGTGAAAAAGAACCTGAAACCGTGT10.9174311926605505No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCCATGATCTCGTATGCCGT10.9174311926605505No Hit
GTACGGGATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGG10.9174311926605505No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAAGGAACCTGA10.9174311926605505RNA PCR Primer, Index 36 (96% over 29bp)
AGAACCTGAAACCGTGTACGTACAAGCAGTGGGAGCACGCTTAGGCTGTCT10.9174311926605505No Hit
GATCATCGAGGCCAAGCCCGATGACATGCGGATCTTCCTCGAAGAAGCCGC10.9174311926605505No Hit
GGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGA10.9174311926605505No Hit
TCTCTCTCTTTCTCTTTGGAAAATGCTTCTTCTTCTTTTGGTGTTAGAGCT10.9174311926605505No Hit
GTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAACC10.9174311926605505No Hit
CTTTATTCCTGCATCTTCTCAATGGTTTCTTCCTTGTATTTGCCCTTTTCC10.9174311926605505No Hit
ATGGAATGGAATGGAATGCAATGGAATGGAACCTGTCTCTTATACACATCT10.9174311926605505No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGCTGTCTCTT10.9174311926605505No Hit
GTTTAGAACCCCGAAATCTGTCTTTTACCAAGACCTCCAAGTAATTCTGAT10.9174311926605505No Hit
AGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG10.9174311926605505No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATATACAAA10.9174311926605505RNA PCR Primer, Index 27 (96% over 28bp)
GTACGGGAAAAAAAGTACTCTGCGTAGAAAAAAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
CTGTTTAACAGCCTGCCCACCCTGGAAACGGCTCAGCCGGAGGTAGGGTCC10.9174311926605505No Hit
GATAAGCCAATGGTGCGAAGCTACCATCTGAGGGATTATGACTGAACGCCT10.9174311926605505No Hit
GTTTTTACCGGTAGCCCAAGTAGTATAGAACTCGGGCTGTCTCTTATACAC10.9174311926605505No Hit
TCTTATACACATCTCCGAGCCCACGACTGTTATACATCTCGTATGCCGTCT10.9174311926605505RNA PCR Primer, Index 39 (100% over 21bp)
TTCATGCAGCTTCCTCTTGATTTAAACACTCATGACACATTGAATGAAATC10.9174311926605505No Hit
AATATGAACAGCAATAAAACTGGCTTGGGATCAAGGTCTATATGAAGGTAT10.9174311926605505No Hit
GAGTACGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCAGCAGGCTC10.9174311926605505RNA PCR Primer, Index 27 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9174311926605505No Hit
ATCCATACCCCAGAACTGGGTCAACACATTCCTTCCCTGAACATCTTCAGC10.9174311926605505No Hit
GGTTTACACAGACAAAGCTGTATCAGACAAAGAAGGAAAGTATAAGTTCAG10.9174311926605505No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTCTGTC10.9174311926605505No Hit
CTACGTTACAAGCTTCTCGGTGGTCTTGCTGTTCGCAGGGCCTGTTATGGT10.9174311926605505No Hit
ACCCTAATAAATCTAGACAAGCACCTCCACCACCAAGCCACGATTACGATC10.9174311926605505No Hit
TTTTTGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTAT10.9174311926605505No Hit
TTGCTATATTGTGCTCGTCTAGTAATACTGTCTCTTATACACATCTCCGAG10.9174311926605505No Hit
GAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGAC10.9174311926605505No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAACATGATCTCGTATGCCGT10.9174311926605505No Hit
ATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAACTAGGCAAAAAAAAAA10.9174311926605505No Hit
AGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGT10.9174311926605505No Hit
GTGGTAAGTAAAATAGGTAAGGCTCTATTTCTGTCTCTTATACACATCTCC10.9174311926605505No Hit
GTACGGGGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGT10.9174311926605505No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10.9174311926605505No Hit
GGTGCTGCCAAAGAACAGGAGCCTCAAAAGACAAAAAAAAAAAAAAAAAAA10.9174311926605505No Hit
TAGCAGAGCAGCCACACGGTCATGATGGCCAGCATGATAGGAAACATTTTG10.9174311926605505No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC10.9174311926605505No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers