FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_80.35100000044d46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_80.35100000044d46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47894
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC1695835.407357915396496No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT25265.274147074790162No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG3990.8330897398421515No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT3520.7349563619660082No Hit
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC3010.6284712072493424Illumina PCR Primer Index 7 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTT850.17747525786110996Illumina PCR Primer Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG610.12736459681797302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC551.8189894E-1244.9989545
TGCCGTC18400.041.697445
TTACTTC250.00208727235.99916527
ATGCCGT21550.035.6024244
CTCCGAG21950.035.36364412
TCCGAGC22000.035.2832713
TCTCCGA22050.035.20326211
CGAGCCC22000.035.18100415
CCGAGCC22000.035.18100414
GAGCCCA21950.035.15863816
AGCCCAC21900.035.1361717
GCCCACG21900.035.1361718
CACGAGA22050.035.10122321
GAGACTC22050.035.10122324
CCACGAG22000.035.0787320
CCCACGA22000.035.0787319
CGTATGC22000.035.0787341
TCGTATG22000.035.0787340
AGACTCT22000.035.0787325
TCTGGAC21950.035.05613329