Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_80.35100000044d46.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 47894 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC | 16958 | 35.407357915396496 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT | 2526 | 5.274147074790162 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG | 399 | 0.8330897398421515 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT | 352 | 0.7349563619660082 | No Hit |
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC | 301 | 0.6284712072493424 | Illumina PCR Primer Index 7 (95% over 22bp) |
TATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTT | 85 | 0.17747525786110996 | Illumina PCR Primer Index 7 (95% over 21bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG | 61 | 0.12736459681797302 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTCTTC | 55 | 1.8189894E-12 | 44.99895 | 45 |
TGCCGTC | 1840 | 0.0 | 41.6974 | 45 |
TTACTTC | 25 | 0.002087272 | 35.999165 | 27 |
ATGCCGT | 2155 | 0.0 | 35.60242 | 44 |
CTCCGAG | 2195 | 0.0 | 35.363644 | 12 |
TCCGAGC | 2200 | 0.0 | 35.28327 | 13 |
TCTCCGA | 2205 | 0.0 | 35.203262 | 11 |
CGAGCCC | 2200 | 0.0 | 35.181004 | 15 |
CCGAGCC | 2200 | 0.0 | 35.181004 | 14 |
GAGCCCA | 2195 | 0.0 | 35.158638 | 16 |
AGCCCAC | 2190 | 0.0 | 35.13617 | 17 |
GCCCACG | 2190 | 0.0 | 35.13617 | 18 |
CACGAGA | 2205 | 0.0 | 35.101223 | 21 |
GAGACTC | 2205 | 0.0 | 35.101223 | 24 |
CCACGAG | 2200 | 0.0 | 35.07873 | 20 |
CCCACGA | 2200 | 0.0 | 35.07873 | 19 |
CGTATGC | 2200 | 0.0 | 35.07873 | 41 |
TCGTATG | 2200 | 0.0 | 35.07873 | 40 |
AGACTCT | 2200 | 0.0 | 35.07873 | 25 |
TCTGGAC | 2195 | 0.0 | 35.056133 | 29 |