FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_8.3510000004444f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_8.3510000004444f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61416
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC850313.84492640354305No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT6601.0746385306760453RNA PCR Primer, Index 44 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT3020.49172853979419046RNA PCR Primer, Index 44 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG1850.3012244366288915No Hit
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC970.1579392992054188RNA PCR Primer, Index 37 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10750.043.1162845
ATGCCGT11600.039.95689444
GTATGCC11850.038.92405342
GAATATC12000.038.812533
TATGCCG11950.038.78661343
CTAGAAT11900.038.76050630
ACCCTAG11900.038.76050627
TATCTCG11900.038.76050636
GAGCCCA11900.038.76050616
AGCCCAC11850.038.7341817
CACGAGA11850.038.7341821
ACGAGAC11850.038.7341822
TCGTATG12000.038.62540
CCTAGAA11950.038.59832829
CCCTAGA11950.038.59832828
TAGAATA11950.038.59832831
GACCCTA11950.038.59832826
AGAATAT12100.038.49173432
CGTATGC12050.038.4647341
CTCGTAT12050.038.4647339