FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_79.35100000044d1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_79.35100000044d1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193732
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC5012925.87543616955382No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT176979.134784134784136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG18490.9544112485288956No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT10840.5595358536535008No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG2200.11355893708834887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC52250.043.49107445
ACTGGTC250.0021032135.9985548
GCCGTCT1700.034.41038545
CCGTCTT352.8124457E-432.14156744
ATGCCGT71000.031.9740744
ATCTCGT74200.030.86803237
TCTCGTA74350.030.83601838
CATCTCG73950.030.78983736
CGTATGC74400.030.78505541
CTCGTAT74400.030.78505539
TATGCCG73900.030.78022443
GTATGCC74100.030.75787542
CACGAGA74600.030.7326821
ACGAGAC74550.030.7231122
TCGTATG74650.030.71209340
GACCATC73850.030.70966733
CCCACGA74750.030.70110919
CCACGAG74750.030.70110920
CGAGACT74150.030.67644923
GGACCAT74050.030.65710632
Produced by FastQC (version 0.11.2)