FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_78.35100000044cf4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_78.35100000044cf4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41773
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC725617.37007157733464No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG3740.8953151557225959No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT3300.7899839609317023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA206.9553754E-445.030
TGCCGTC8000.041.62500445
AGCCCAC8550.040.2631617
GCCCACG8500.040.23529418
CCCACGA8500.039.9705919
CGAGACT8500.039.9705923
ATGCCGT8350.039.88023844
CCACGAG8550.039.73684720
ACGAGAC8550.039.73684722
GAGACTC8450.039.67455724
TCTGGAC8350.039.61077529
CATCTCG8350.039.61077536
GACCATC8300.039.57831233
GAGCCCA8700.039.56896616
ACTCTGG8450.039.40828327
AGACTCT8450.039.40828325
GACTCTG8450.039.40828326
CTCTGGA8400.039.37528
GGACCAT8400.039.37532
CGAGCCC8800.039.37515
Produced by FastQC (version 0.11.2)