Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_77.35100000044cda.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 178341 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC | 113125 | 63.4318524624175 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT | 13693 | 7.677987675296202 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG | 4858 | 2.7239950432037503 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT | 696 | 0.3902635961444648 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCCTGGACCATCTCGTATGCCGTC | 358 | 0.20073903364902068 | No Hit |
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC | 225 | 0.12616280047773648 | Illumina PCR Primer Index 7 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCTGTC | 196 | 0.10990181730505043 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCTGGAC | 40 | 6.7611836E-9 | 44.99804 | 29 |
TGCCGTC | 11900 | 0.0 | 44.090515 | 45 |
CGAGACC | 80 | 0.0 | 42.18566 | 23 |
CCCTGGA | 55 | 6.002665E-11 | 40.907307 | 28 |
GAGACCC | 80 | 0.0 | 39.373283 | 24 |
ATGCCGT | 13410 | 0.0 | 39.12581 | 44 |
GACCCTG | 75 | 0.0 | 38.9983 | 26 |
AGACCCT | 75 | 0.0 | 38.9983 | 25 |
CGTCTTC | 35 | 6.220507E-6 | 38.569748 | 45 |
TCCGAGC | 14065 | 0.0 | 37.511692 | 13 |
TCTCGTA | 14035 | 0.0 | 37.495693 | 38 |
CCACGAG | 14080 | 0.0 | 37.487713 | 20 |
CTCCGAG | 14080 | 0.0 | 37.487713 | 12 |
CCCACGA | 14075 | 0.0 | 37.485046 | 19 |
ATCTCGT | 14010 | 0.0 | 37.482307 | 37 |
CACGAGA | 14090 | 0.0 | 37.47707 | 21 |
CTCGTAT | 14030 | 0.0 | 37.46095 | 39 |
TATGCCG | 14025 | 0.0 | 37.458256 | 43 |
GCCCACG | 14080 | 0.0 | 37.455753 | 18 |
ACGAGAC | 14095 | 0.0 | 37.44782 | 22 |