FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_77.35100000044cda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_77.35100000044cda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178341
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC11312563.4318524624175No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT136937.677987675296202No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG48582.7239950432037503No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT6960.3902635961444648No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGACCATCTCGTATGCCGTC3580.20073903364902068No Hit
ATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCTTC2250.12616280047773648Illumina PCR Primer Index 7 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCTGTC1960.10990181730505043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGAC406.7611836E-944.9980429
TGCCGTC119000.044.09051545
CGAGACC800.042.1856623
CCCTGGA556.002665E-1140.90730728
GAGACCC800.039.37328324
ATGCCGT134100.039.1258144
GACCCTG750.038.998326
AGACCCT750.038.998325
CGTCTTC356.220507E-638.56974845
TCCGAGC140650.037.51169213
TCTCGTA140350.037.49569338
CCACGAG140800.037.48771320
CTCCGAG140800.037.48771312
CCCACGA140750.037.48504619
ATCTCGT140100.037.48230737
CACGAGA140900.037.4770721
CTCGTAT140300.037.4609539
TATGCCG140250.037.45825643
GCCCACG140800.037.45575318
ACGAGAC140950.037.4478222