FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_76.35100000044cb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_76.35100000044cb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374697
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC324298.654726352225932No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT112382.9992233724849675No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCG14980.3997896967416339No Hit
GAGTCATTGTGGTTAGGGATTAGAGTCTCAACATCTCCCAAGTAGCTCCCT8910.23779213604592514No Hit
AAAGAAGCAGTTGAGCAGAGAAAAGCTGAGAAGAGAGGCTTGAAATCTGAT7230.19295590837396617No Hit
GATTCAAGAGTCTTGAGTATCTCAATGAAATCCTTCTTCCCTGTCTCTTAT6600.17614232299698157No Hit
GGAAGAAGGATTTCATTGAGATACTCAAGACTCTTGAATCTGAGCTTGGAG6570.175341676074268No Hit
GTCATTGTGGTTAGGGATTAGAGTCTCAACATCTCCCAAGTAGCTCCCTCT6300.16813585376984605No Hit
CTCCATTTCTTCACGTTCTTCTTCATCTCTTTGCTCCTCTCTCCTTCCATC5750.1534573268534309No Hit
GGAAGAAGGATTTCATTGAGATACTCAAGACTCTTGAATCCTGTCTCTTAT4950.13210674224773616No Hit
GGTTTGTCTCCAAGCTCAGTTTCAAGAATCTTGAGTGCTTCTATATACTCT4890.13050544840230907No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTCT4830.12890415455688195No Hit
CTCCAAGCTCAGATTCAAGAGTCTTGAGTATCTCAATGAAATCCTTCTTCC4260.11369186302532445No Hit
GCTGTAATATCCAGTCATGGCAATGTCTACGAACCCGAAGGTATCTCCACC3830.10221592379976355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC34050.042.8831745
AGCCCAG356.1993887E-638.6054341
GCCCAGA356.2221716E-638.584822
GCCGTCT1300.036.34421545
CGGCAAA250.00210585235.9980779
CGGATGC250.00210585235.99807716
AGCCCTC702.1827873E-1135.35525516
TCAGCCC702.1827873E-1135.35525514
CAGCCCT754.7293724E-1132.99823815
GAGAGGC754.7293724E-1132.99823833
ATGCCGT46250.031.57128544
GGAGTCC651.39898475E-831.15218431
GCCCTCA801.0004442E-1030.93584817
CTAGTAC950.030.7960514
GCTCAGT950.030.7878314
TATGCCG47900.030.48375943
CATCTCG47900.030.38981836
CTGGACC48100.030.21668430
GTATGCC48200.030.20067242
TGGACCA48200.030.10731531
Produced by FastQC (version 0.11.2)