FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_75.35100000044c97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_75.35100000044c97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC95.294117647058823No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGT31.7647058823529411No Hit
CAATGGTTATGGAGAAGAAAAGTGATGGCAGTTAGAAGATGTGCATTGATT10.5882352941176471No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGTCTCTTATA10.5882352941176471No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAGTCTTAGAT10.5882352941176471Illumina PCR Primer Index 8 (96% over 27bp)
GCTCCCGAGAGAGCCGTTCCCAGTCCGTCCCCCGGCCAGCACGCGGCGACC10.5882352941176471No Hit
GCCTTGGAGAAGGCACAATACCCAGTGGTTGATCGGAATCCTGCTTTTACT10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACCAA10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
TACCGAGACCCAGCAAAGCTCCGACCATTGTCGCACTTATAGGCATCGTTT10.5882352941176471No Hit
CAAGGAGAATGAAGGTGTGAAGTTTGTGAGGATGGAGAGAAGAATGGGTAA10.5882352941176471No Hit
AAGTATCTGAAACTGTTTGAGACGATAACACAAGTGAAGAACAAGAACAAG10.5882352941176471No Hit
GCTCACACTCGAACCCTTCTCAGAAGATCAACTGTCTCTTATACACATCTC10.5882352941176471No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.5882352941176471No Hit
TTCTTTGGCTTCTTTTTTAGAATCTTCTGTGTCTGTCTCTTATACACATCT10.5882352941176471No Hit
GAGCTGACTTCTTGCTAGCATTCGGAGCCTTGACATAAGCAACTAGGGAAG10.5882352941176471No Hit
TGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTCCGAG10.5882352941176471No Hit
GGCTATTAGCATCTGACGATTTAAACAAGGTATCAAACTGTCTCTTATACA10.5882352941176471No Hit
TAATGATAGTGTGTCGAAACACACTGGGTCTGTCTCTTATACACATCTCCC10.5882352941176471No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACAGTCCCT10.5882352941176471No Hit
CGATGGGGATAGCCAGCCCGGGCTTAATCCTGTCTCTTATACACATCTCCG10.5882352941176471No Hit
TTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCTGT10.5882352941176471No Hit
CCCGTACTCTGCGTTGATACAACTGCTTCCCGTACTCTGCGCTGATACTGT10.5882352941176471No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTAAACACC10.5882352941176471Illumina PCR Primer Index 8 (96% over 27bp)
ATTCAGGATTGAAATGATGTAGTCCTCCATGAAGAATTCAAAGCAAGAGTT10.5882352941176471No Hit
ATATAGATGTGTCTCCCTCTTGTAGTAAGGAAAAGAATGGATTCTAACCAG10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAATCAAC10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
GTTGTATTTACCACTCTTCATAACAAGACGAAGACTGTCTCTTATACACAT10.5882352941176471No Hit
TTAGAGAGCTGTATGCTTCACATGCATAAGCATCCTGGTTTCTTTATTCCC10.5882352941176471No Hit
GGTATCAACGCAGAGTACGGGAGTGGTATCTGTCTCTTATACACATCTCCG10.5882352941176471No Hit
GGATGGGATGGAAAATAATTTTACATAGCTACAGACCTGTCTCTTATACAC10.5882352941176471No Hit
CTACTTAACCGTCATCTCACAAATTGGGAACTTAGGACCACTTATAGTCAC10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCACAACATA10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA10.5882352941176471No Hit
TCTCATTGTGTGTGTGTGTGTGTGTGTGTGCTTATGAAATCAACTCTCTTT10.5882352941176471No Hit
AAAAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC10.5882352941176471No Hit
AGTACGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA10.5882352941176471No Hit
GCTTACTATAGTCAAACTCTAAGTACTTCAGCCAGAAACTCTGTCTCTTAT10.5882352941176471No Hit
CTCTNTGATAGACATCAACTCAGGGAACTCAGTCTTCAACTTAGTTGCATC10.5882352941176471No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10.5882352941176471No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10.5882352941176471No Hit
GCATTAGAAAATGCTAAGGCATTAGAAAAGTAGTTTCTGTCTCTTATACAC10.5882352941176471No Hit
GGACCTATGAAGATGCGCCGTCGTGATTTCCTTGCGCCTGTCTCTTATACA10.5882352941176471No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACGGTGGATGC10.5882352941176471No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC10.5882352941176471No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAAAGCTACA10.5882352941176471Illumina PCR Primer Index 8 (96% over 27bp)
CCTGTATATCTTTCCAGAAAGTCAGTGTACATACAAGAACTAATGCTGTCC10.5882352941176471No Hit
CCCGTTATGCGGTGCGGTGATGTGTTCCTCGCTACCGCAGCATTAGGGGCC10.5882352941176471No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATGGCATGG10.5882352941176471RNA PCR Primer, Index 36 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCCGGACCATCTCGTATGCCGTC10.5882352941176471No Hit
GGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAGTACCTGTCTCTTATAA10.5882352941176471No Hit
CTGCGTTGATACCACTGCTTCCCGTACCTGTCTCTTATACACATCTCCGAG10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTCTCCTAACC10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTTTAACCCCCCCCCGC10.5882352941176471No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTCGTTTAACGCTTCCCCCC10.5882352941176471No Hit
CGTCAATGAGCAAAGGTATTAACTTTACTCCCTGTCTCTTATACACATCTC10.5882352941176471No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGCTTTGA10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAGTACAGG10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
ACGACGGCAAGGCGATTGCTCATACCATCAGATCAGAGATCGCCGAGGAAG10.5882352941176471No Hit
GATAAGGGGATCGTTGTAGATTAGCTGAATGGATCCAGTGTAGTAACAGGG10.5882352941176471No Hit
CATCAGGTATATGTAAAGACCAGAGCGCATTAGAGAAGTAATAAATGAGAG10.5882352941176471No Hit
ATTGTAGTTAAGGAAAAACTGCTGAGATTTCCCAGATTCGTATACCTGTCT10.5882352941176471No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC10.5882352941176471No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC10.5882352941176471No Hit
ATATAAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATAGCTAAAAC10.5882352941176471Illumina Paired End PCR Primer 2 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGATACTCTGGACCATCTCGTATGCCGT10.5882352941176471No Hit
GTATCAACGCAGAGTACTTTTCTGTCTCTTATACACATCTCCGAGCCCACG10.5882352941176471No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10.5882352941176471No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACC10.5882352941176471No Hit
ACCCTAAAACTAATATGTTGAATAAGAAACATTAGAAGCACACCCGATGAA10.5882352941176471No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG10.5882352941176471No Hit
GTATTAGACACATTCAGCTTGACAGGAAGCAAATCTGTCTCTTATACACAT10.5882352941176471No Hit
TTAAAAAGTGGGCAAATGACAGATACTTCTCAAAAGAAGACATACAAGCTG10.5882352941176471No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGCTGTCTCTT10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAAAAAACA10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA10.5882352941176471No Hit
GTACTTTTTTTTTTTTTTTTTTTTCTTTGATACCCCGTACCCCTGTCTCTT10.5882352941176471No Hit
TCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT10.5882352941176471No Hit
GCTCAGACAAGAAACACGGAAGTACTTTCCGCAAGCTGTTCCCAAATCAAC10.5882352941176471No Hit
GTGTATGGTGTGTTCAGCATTGCTGAATTCTTCTCTGATATCTTCCTGTCA10.5882352941176471No Hit
GCGTTGATACCACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACT10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGACCATCTCGTATGCCGTC10.5882352941176471No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC10.5882352941176471No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAATTACTCTGCCCTTT10.5882352941176471No Hit
GGACATAGTGATCCGGTGGTTCTGAATGGAAGCTGTCTCTTATACACATCT10.5882352941176471No Hit
TCCCTACTATGCCTAGAAGGAATAATACTATCGCTGTTCATTATAGCTACT10.5882352941176471No Hit
ATACTGTAGGAACAAGCATGATCTTGTTACTGCTGTCTCTTATACACATCT10.5882352941176471No Hit
ATCTAAAACTGTTCTTATTCCTCAGGTTGTTTTGTTCCTTGGTGAACTGCA10.5882352941176471No Hit
GAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTCTCCCG10.5882352941176471No Hit
TGGTTAGTATTATTATGCATTAAGAAATCTTCACCGTTGCTGTCTCTTATA10.5882352941176471No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCTGTC10.5882352941176471No Hit
CTGTTAGCTGCTTTGTTGTTTTCACAAGTTACTTCAATCTTCTTATGAAAC10.5882352941176471No Hit
CCATTCTACTCTATGTGAATGAGTCAGTCCAAACCTGAAATATCTCTGTCT10.5882352941176471No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAAACGTCC10.5882352941176471Illumina PCR Primer Index 8 (96% over 27bp)
ACGTATATGTGCTCATTAAAACCAGTGAATCTGTCTCTTATACACATCTCC10.5882352941176471No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA10.5882352941176471No Hit
ATCAAGCAGAGCAAAAAAAAAAAAAAAAAAATACCTCTCTGTCTCTTAAAA10.5882352941176471No Hit
ATTCCAAGATCTTGATTTCACTTTCTTGGAGATGAATCCTTTCACTCTAGT10.5882352941176471No Hit
CTACTGGAAACGGTAGCTAATACCTGTCTCTTATACACATCTCCGAGCCCA10.5882352941176471No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG10.5882352941176471No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGTCTTGCTGTCTCTTA10.5882352941176471No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACGAACCA10.5882352941176471Illumina PCR Primer Index 8 (96% over 27bp)
TTGCAAAAGGCTGAGAAAGGAATTGAGGGATAGTGAAAACAAAAAATAAAC10.5882352941176471No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT10.5882352941176471No Hit
ACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTGGGCCATCCCGTATGCCGGC10.5882352941176471No Hit
CAACCAGAGTACTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACCCTGTC10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGTGTGCATCTCGTATCCCGTC10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACTATCTCGTATGCCGTC10.5882352941176471No Hit
GTTTCATTCTTCGTAGACAAGTCAACAGCAACATAATGACGTCATTTCGAT10.5882352941176471No Hit
CTTATACACATATCCGAGCCCACGAGAAATGCGTTATCTCGTATGCCGTCT10.5882352941176471No Hit
TATCAACGCAGAGTACGGGGTATCCTGTCTCTTATACACATCTCCGAGCCC10.5882352941176471No Hit
AACAACAAGAGATAAGAAAAAAGAAGCCAAACATCTTAGGCTGTCTCTTAT10.5882352941176471No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACCGCCTCT10.5882352941176471No Hit
AAACGATGATGAAGAAGATGATGATGACGTTGGGAATGAGTATGAGAACTG10.5882352941176471No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCTGTCTCTTATACACATCTCC10.5882352941176471No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA10.5882352941176471No Hit
AGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCCG10.5882352941176471No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATACCACCCC10.5882352941176471No Hit
GGGAGGTATCAACGCAAAAAAAAAAAAACTGTCTATTATACACATCTCCGA10.5882352941176471No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.5882352941176471No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAGATCCAAT10.5882352941176471RNA PCR Primer, Index 36 (96% over 29bp)
GGATCATTCAGTTCAGTAACAACAATAAATTCGCCCTGTCTCTTATACACA10.5882352941176471No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACATAAA10.5882352941176471Illumina PCR Primer Index 8 (96% over 27bp)
TATCAACGCAGAGTACGGAGACACGGTCCAGACTCCTACGGGAGGCAGCAG10.5882352941176471No Hit
ATATACTTTTGCTTGGTTCATTTTTTTTCCCTTTTAGTTAAGCATGACTTT10.5882352941176471No Hit
CTTCAAAGTTGTCTATGTTCAAGGAAGTTTTACCGAAAACAGGACAACAAT10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGAGAGATGAT10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.5882352941176471No Hit
TGTCTTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGTCATCACATTC10.5882352941176471TruSeq Adapter, Index 20 (96% over 25bp)
GGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAA10.5882352941176471No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCA10.5882352941176471No Hit
GTGTCCCCGGAGCTTGTGTGCGATACAGAGAGCACCTCGGAAGCTGAGGCA10.5882352941176471No Hit
CCACTGCTTCCCGTACTCTGCGTGATACCTGTCTCTTATACACATCTCCGA10.5882352941176471No Hit
ATCACCAAAACATTCTTTTGCCCACAGCATCTACTGTCTCTTATACACATC10.5882352941176471No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTCTGTCG10.5882352941176471No Hit
TTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC10.5882352941176471No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTTCGTATGCCGC10.5882352941176471No Hit
CATTTTAATCTAAAAGCATTGCCCTTCTATTGGTATTAATTCGGGGCTCTG10.5882352941176471No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCT10.5882352941176471No Hit
CACTAACACTAACACTAACACTAACACTAACACTAACACTAACACTAACAC10.5882352941176471No Hit
GTAGTCCATATTGCTATCTTATCACCTTTAGCTCTAACATTAACAACAGCG10.5882352941176471No Hit
CCATTCTGTTGTCAAAATCTTCACCTCCCAAATGAGTATTTCCAGCAGTAG10.5882352941176471No Hit
TTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCGACCTCTACTGCCTCT10.5882352941176471Illumina Single End Adapter 1 (95% over 22bp)
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAACTGTC10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAGCGAACAAG10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
CACAAATACACACACACACACATATCCACACACATACAAACACACACATGC10.5882352941176471No Hit
CCACATGAAACATCCTATCATCTGTAGGCTCATTCACTGTCTCTTATACAC10.5882352941176471No Hit
ATCTTAGCCATGATCAAACTCTTCAATTTCCCGTACTCCTGTCTCTTATAC10.5882352941176471No Hit
GTATGTTCAGCAGCATCTTTCCTCTCTTGACCAGATTTGAACTCAGCTAAG10.5882352941176471No Hit
TACTACTGCTTCCCGTACTCTGCGTTGATACAACTGCTTCCCGTACTCCTG10.5882352941176471No Hit
GATCTCGCTCTTTCCAAAAACCAAAAGACAACAAAAGATGATAAAACTCCA10.5882352941176471No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTCTGTCTCT10.5882352941176471No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC10.5882352941176471No Hit
CTTGTACATAGATAACCACACAAGACAATTGTCTTGTTCTAACCCTGTCTC10.5882352941176471No Hit
ACACTACAAGCTTCTTGATCCCTGCAATGAGAAACATTCACAAAGCTCTTA10.5882352941176471No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA10.5882352941176471RNA PCR Primer, Index 27 (96% over 28bp)
GCTCGAAGCTTTCTCAACCTCTTTGTCCCAATCAGTTCGTAAAGTGACAAT10.5882352941176471No Hit
GTACCAACCACTACAAAAACATACCAAATTGTAAAGACCATTGACACTATG10.5882352941176471No Hit
AATTAAGGGTGGTGAACAGGGTTGGGCTGCTCATCCACTTTGGGCAGATGC10.5882352941176471No Hit
GACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACAC10.5882352941176471No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers