FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_72.35100000044c3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_72.35100000044c3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167396
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC6281337.52359674066285No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT130477.794093048818371No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG118997.108294104996535No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT5700.3405099285526536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC65650.044.28027745
GCCGTCT1050.040.71428745
TATCTCG403.4346704E-739.37536
ATGCCGT78650.036.9612244
ATCTGAA250.002101979736.01
TATGCCG89550.032.4874443
CGTATGC90550.032.42683441
GTATGCC89850.032.4040142
CTCGTAT90550.032.3771439
TCTCGTA90650.032.36624538
TCGTATG90600.032.35927240
ATCTCGT90550.032.3522937
GATCTCG90050.032.28206336
TATGATC90200.032.1784933
ACATCCT352.8101576E-432.14285731
TGATCTC90450.032.139335
ATGATCT90350.032.1250734
ACGAGAC91500.032.06557522
GAGACTC91450.032.05850224
CACGAGA91400.032.05142221
Produced by FastQC (version 0.11.2)