FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_71.35100000044c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_71.35100000044c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164818
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC6994742.438932640852336No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT2079312.615733718404542No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG32031.9433557014403766No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT9990.6061231176206482No Hit
ATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCTTC1810.10981810239172907Illumina PCR Primer Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC351.2035343E-744.99969545
TGCCGTC75750.043.00961345
ACTCTCC250.002101956235.9997568
GCCGTCT3300.034.7724945
ATGCCGT98950.032.925544
TATCTCG352.810084E-432.1426436
TCCGAGC103400.031.7696113
CCCACGA103500.031.76065419
GAGCCCA103600.031.75171716
GCCCACG103450.031.73250818
AGCCCAC103600.031.7317
CGAGCCC103750.031.72749515
CACGAGA103550.031.7235921
CCGAGCC103650.031.71469114
ACGAGAC103600.031.7082822
CCACGAG103600.031.7082820
AGACTCG103300.031.6914625
CGAGACT103400.031.6825723
CTCCGAG103550.031.68013412
ACTCGCT103250.031.64143427
Produced by FastQC (version 0.11.2)