FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_7.35100000044418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_7.35100000044418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15432
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC377924.488076723691034No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT3242.0995334370139966RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG1210.784085018144116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4250.044.47058545
ATGCCGT4400.042.95454444
TCTCGTA4550.042.03296738
ACGAGAC4550.042.03296722
GTATGCC4500.042.042
TATGCCG4500.042.043
TATCTCG4500.042.036
CGTATGC4500.042.041
TCGTATG4500.042.040
AGACCCT4500.042.025
CTCGTAT4500.042.039
GAGACCC4500.042.024
GACCCTA4450.041.9662926
AGAATAT4400.041.9318232
ACCCTAG4400.041.9318227
GAATATC4400.041.9318233
CTAGAAT4400.041.9318230
CCTAGAA4400.041.9318229
CCCTAGA4400.041.9318228
TAGAATA4400.041.9318231
Produced by FastQC (version 0.11.2)