FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_69.35100000044bdb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_69.35100000044bdb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324000
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC8390825.89753086419753No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT138064.261111111111111No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG55561.7148148148148148No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT11330.3496913580246913No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC8380.25864197530864197No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT8330.2570987654320988No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT6660.20555555555555557No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT6630.20462962962962966No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA6480.2No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC6450.19907407407407407No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCT5900.18209876543209877No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT5860.1808641975308642No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA5530.170679012345679No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA5500.1697530864197531No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC5470.16882716049382718No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT5440.16790123456790124No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA4950.1527777777777778No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTC4680.14444444444444443No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT4530.1398148148148148No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA4470.13796296296296295No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4430.13672839506172837No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA4230.13055555555555554No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4230.13055555555555554No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC4010.12376543209876543No Hit
AAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTT3730.11512345679012347No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT3580.11049382716049382No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC3530.10895061728395063No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA3490.10771604938271606No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT3460.10679012345679012No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG3460.10679012345679012No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT3400.10493827160493827No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3300.10185185185185185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC83150.044.56612845
GCCGTCT750.041.99913445
GACCCGC356.235292E-638.57063326
AGACCCG356.235292E-638.57063325
ATGCCGT98550.037.60196744
TAAACGA250.002105093335.9992629
CCGCTAT250.002105093335.9992629
ACGCTTT250.002105093335.999266
TATGCCG104600.035.448643
GTATGCC104950.035.33038342
CGTATGC106000.035.17144841
TCGTATG106100.035.15950440
ATCTCGT106050.035.1124337
CTCGTAT106200.035.1052139
TCTCGTA106400.035.0603738
GATCTCG105700.035.0371236
TGATCTC105900.034.992235
CTATGAT105900.034.9284632
TATGATC105850.034.92370633
GCTATGA106200.034.8933531
Produced by FastQC (version 0.11.2)