FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_68.35100000044bb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_68.35100000044bb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105767
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC63015.9574347386235775No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC5440.5143381205858161No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5290.5001560032902512No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT3930.3715714731437972No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA3410.322406799852506No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA2870.27135117758847277No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG2800.2647328561838759No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA2590.2448778919700852No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG2550.24109599402460125No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCC2490.23542314710637532No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA2480.23447767262000435No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.2306957746745204No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2390.22596840224266548No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2340.22124102981081056No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG2270.21462270840621367No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT2240.2117862849471007No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.20327701456976183No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA2130.20138606559701985No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC2110.1994951166242779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.19760416765153593No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1920.18153110138322917No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT1890.17869467792411622No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC1830.1730218310058903No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT1830.1730218310058903No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCT1820.1720763565195193No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC1680.15883971371032551No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG1680.15883971371032551No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC1590.15033044333298665No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC1490.1408756984692768No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.13709380052379289No Hit
CTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTT1440.13614832603742189No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTC1430.13520285155105088No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTAT1420.1342573770646799No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1380.13047547911919596No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA1330.12574810668734104No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTC1320.12480263220097006No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1300.12291168322822808No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCCTGTCTCTTATACACATCTCC1290.1219662087418571No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.1219662087418571No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT1280.12102073425548612No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCCTGTCTCTTATACACA1270.12007525976911514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.12007525976911514No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGCTGTCTCTTAT1250.11818431079637316No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA1220.1153478873372602No Hit
GGTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCC1210.11440241285088922No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.11440241285088922No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGCTGTCTCTT1150.10872956593266331No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.10778409144629232No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT1120.10589314247355035No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT1120.10589314247355035No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1110.10494766798717936No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACAT1110.10494766798717936No Hit
GCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA1100.10400219350080839No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGTA253.8710677E-544.991492
CCTGTAT207.010766E-444.9914863
TGCCGTC7150.044.36223645
ATGCCGT7850.040.4063744
TATGATC8200.037.87660233
CTATGAT8200.037.87660232
CGTATGC8450.037.803541
TATGCCG8350.037.71741543
CGCTATG8250.037.64704530
GCTATGA8250.037.64704531
ATCTCGT8500.037.59890437
TCGTATG8500.037.58112740
CTCGTAT8500.037.58112739
GACTCGC8450.037.55503526
GTATGCC8400.037.4929142
GATCTCG8500.037.33412636
AGACTCG8500.037.33412625
GAGACTC8500.037.33412624
ACTCGCT8400.037.2427127
ATGATCT8400.037.2427134
Produced by FastQC (version 0.11.2)