FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_67.35100000044b98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_67.35100000044b98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC97.377049180327869No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT32.459016393442623No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT21.639344262295082No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT21.639344262295082No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA10.819672131147541No Hit
ATGTAATCTACTCAGGTTATGAATGCACTAAAATGCTGTCTCTTATACACA10.819672131147541No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC10.819672131147541No Hit
TAATAATACTCCCTCTGAATGCTTTTCAAAATGGATGTCCCTAAATGCTGT10.819672131147541No Hit
CAGAAAAGACAACAACAGTGAAATTCAACAGAAGAAAAGTAATGGACTCTG10.819672131147541No Hit
ATACATAACTTGGCAACTAACTTACGGTAGTATGCATCTGTCTCTTATACA10.819672131147541No Hit
ATGGGAAACAGGTTAATATTCCTGTACTTGGTGTTACTGCTGTCTCTTATA10.819672131147541No Hit
AAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACG10.819672131147541No Hit
GTAGTTTCCTATTAAACTATTTCCTCACAAAATGCATTCTGTCTCTTATAC10.819672131147541No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA10.819672131147541No Hit
TCACACGAGGGTTCGGGCCCGTAAGGCTGGCGAAAGAAAGGGCAGCGGAAG10.819672131147541No Hit
TCATTAACCTATGGATTCAGTTAATGGTAGCTGTCTCTTATACACATCTCC10.819672131147541No Hit
GTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTT10.819672131147541No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTTGCCTCATTAACCTATGGATTCA10.819672131147541No Hit
ATACCACTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCT10.819672131147541No Hit
ACACATATCAGAAGAGAAGCCATTCTCCAGTAGTAAGTAACTCTGTCTCTT10.819672131147541No Hit
CACCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC10.819672131147541No Hit
GGGGACACCCAGTGTGTTTCGACACACTATCATTAACCTCTCTCTTATACA10.819672131147541No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAGGAACTCCTGGT10.819672131147541No Hit
AATGTCTTCTGACCTGATTTTTCAAAGATTGGTTGATACTTCTTCTGTCTC10.819672131147541No Hit
GATTATCTGTAAGCATTTCTCTATTCAGATCCATAATCCAAGTGCTCTCTG10.819672131147541No Hit
GTTATTTGGTAGAGTGATAAAAGCAAGCATTGCTATTGACAATGGAAGAGC10.819672131147541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATCACCAAA10.819672131147541Illumina PCR Primer Index 8 (96% over 27bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATAAGCCTT10.819672131147541Illumina PCR Primer Index 8 (96% over 27bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA10.819672131147541No Hit
AATGTTATCTCCTCTGAGAAGCCTTTCTTAATTCTCTCAGGCAGGATTTTT10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCCTATGATCTCGTATGCAGTC10.819672131147541No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGGG10.819672131147541No Hit
GAACATCACGCTGGTGCTGCCGTTCGCGGCGGGAAGCGGCACCGACAGCAC10.819672131147541No Hit
AAAAAGTACTCTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC10.819672131147541No Hit
ATGTGTTTACTTGCTCGTTATATGTGGATCTAAGTCTGTCTCTTATACACA10.819672131147541No Hit
GCATAAGAGATTCGAATAAGTACACGATGGCCCTAATGGTGAACGCTAAAG10.819672131147541No Hit
GCTTACAGTAAAACCCTGTTTTAGGTATCACATAGTCTATACCAACAGGAA10.819672131147541No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10.819672131147541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATACAACACGA10.819672131147541RNA PCR Primer, Index 27 (96% over 28bp)
TACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10.819672131147541No Hit
CCAAAACAAGGGCACACTTCATAGCTTTGATGGTGTTGAAATTGTCTGGTC10.819672131147541No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT10.819672131147541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCCAAAAAGA10.819672131147541RNA PCR Primer, Index 27 (96% over 28bp)
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGC10.819672131147541No Hit
ATATAAATCTCGTATGCCGTCTTCCGCTTGCAAAAAAAAATCTCTTATAAA10.819672131147541Illumina PCR Primer Index 4 (96% over 25bp)
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAAAAAGATA10.819672131147541Illumina PCR Primer Index 8 (96% over 27bp)
GTTACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAACTGTCTCT10.819672131147541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGGTTTTATACCC10.819672131147541No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCC10.819672131147541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACCACCTCA10.819672131147541Illumina PCR Primer Index 8 (96% over 27bp)
ATATTAAACCAGACAAAAAAGTGTGAAAAGCGAAAAAAACATACAAAACAT10.819672131147541No Hit
TTATAGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAGAGCTTCGCTTGC10.819672131147541No Hit
GTGTTTCGACACACTATCATTAACTGAATCCCTGTCTCTTATACACACCTC10.819672131147541No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT10.819672131147541No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCT10.819672131147541No Hit
ATCTTGCACAGCACTGCTAATGTAAATTTCAGTTGTTTTTCCCTCTAGGAC10.819672131147541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAACAC10.819672131147541Illumina PCR Primer Index 8 (96% over 27bp)
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATA10.819672131147541No Hit
ATATTTTTGTCTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGT10.819672131147541No Hit
GGCCATATTCTTTTCTCCTTTTTTCTAAAAAAGAAAAAACCCACATCTTTT10.819672131147541No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT10.819672131147541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAGACACCAA10.819672131147541RNA PCR Primer, Index 27 (96% over 28bp)
TTACTAAACATATCATTGATGCCTTGATGCAATCTTAAGAAGACTGTCTCT10.819672131147541No Hit
GTTTTCCACTTCAAACTTCTATCTTCTCCAAATTGTTTCATCCTACCACCT10.819672131147541No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGAT10.819672131147541No Hit
AGTCTGGACCGTGTCTCAGTTCCAGTGTGACTGGTCATCCTCTCAGACCAG10.819672131147541No Hit
GAATGATCAACTTGCTCTCTTGATTTCCATAAGCTAGGATCTGTCTCTTAT10.819672131147541No Hit
CGGAATTGCTTGTCGATTTATTAAATATAACGCAACGAAATAACTGTCTCT10.819672131147541No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCG10.819672131147541No Hit
GGAGTATTGAGTACCTGGATGTATAAGTGTTACCTCTGTCTCTTATACACA10.819672131147541No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCGTTTATCTCGTATGCCGTC10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC10.819672131147541No Hit
CTACAGAGACTTCCACAAAAACTTTTGAATGATGTGAAACTGTCTCTTATA10.819672131147541No Hit
AGACAGAGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCTGGCCCCAA10.819672131147541No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATCTGGCCCT10.819672131147541No Hit
ATCTTAGATTGCCTACATGTAAGGTCATATCATCCTGTCTCTTATACACAT10.819672131147541No Hit
TGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGAT10.819672131147541No Hit
CATTTGAAGCCTGTACAAAAGCTTATCCCTGTAACACATGTGCCATAATAT10.819672131147541No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC10.819672131147541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAACCACTT10.819672131147541RNA PCR Primer, Index 27 (96% over 28bp)
AAAAAAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG10.819672131147541No Hit
CTTGATTCTTGAGTCATGATGTTATTTTCTTCTAATTTTTCCGAACCGTCT10.819672131147541No Hit
ATTCTGGAGAGAACTGAGTGCAACACCAGAGTTAACTGAAGGAGTTGTTGG10.819672131147541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATATACAAAGAC10.819672131147541Illumina PCR Primer Index 8 (96% over 27bp)
GCGCAGATCCAGCGTCAGCTGCGCGGTGTCTGGCGCGGCGCGAGTGCCGGC10.819672131147541No Hit
TCTTATACACATCTCCGAGCCCACGAGATTCGCTATGATCTCGTATGCCGT10.819672131147541No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTCTGCCCCTTAT10.819672131147541No Hit
ACCTTAAGCTCACCTTCTTTACTTGTATCAAATGATGACCTGTCTCTTATA10.819672131147541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATAACCGC10.819672131147541RNA PCR Primer, Index 27 (96% over 28bp)
AATTAGGAGGACATTAACAATTAAGTGTTAATGCGAACACCGTTAACAGTA10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCTAGTATCTCGTATGCCGTC10.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCCGTTATCTCGTATGCCGTC10.819672131147541No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATATAATTTTA10.819672131147541RNA PCR Primer, Index 27 (96% over 28bp)
CCCCATTTCCAGTCTGATTATACAAGTGCTAAGTGGCAGAAAGGTCTGGAA10.819672131147541No Hit
GTGCAGTTCCAGTAGTGACTGATTCACCTTTTTTTCCAAATGTAATGCACA10.819672131147541No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA10.819672131147541No Hit
ATGCGCGTGTGCTGATCAGGCAACGTCACATTCGTGTTCGTAAGCAAGTAG10.819672131147541No Hit
TCGGTAAAGTTGGTATGAGGTATTTCCACAAGCTGAGGAACAAGTTCTTTT10.819672131147541No Hit
TTTTTACTTTTCTAACGAAGACATCATGGAGAGGATAAATAGCTGTCTCTT10.819672131147541No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAAATAGACAG10.819672131147541RNA PCR Primer, Index 36 (96% over 29bp)
CGATGGAACCTTAGCTATTCCCATAGCCTTAGCCTTGAGTGTAAAAGACTG10.819672131147541No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAACAAAA10.819672131147541Illumina PCR Primer Index 8 (96% over 27bp)
GATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGACGATT10.819672131147541No Hit
TGATCACATTGACGTAATGTTGACAGGACATTGAATGACAATACCTGTCTC10.819672131147541No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10.819672131147541No Hit
TTTCTTGAACAACTTTTTCTCCGTTAAGAATTTGAACTTTCACTGATGTTG10.819672131147541No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGC10.819672131147541No Hit
ATACTGGGCCCTATTCAGTGGCAGCTTCTTGTTCCATAGGATTAAGGAAGA10.819672131147541No Hit
GGGAAACCCAGTGTGTTTCGACACACTATCATTAACCTGTCTCTTATACAC10.819672131147541No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers