FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_65.35100000044b55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_65.35100000044b55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC67.0588235294117645No Hit
GTTTTATTTTCATTTGTCCCAAGACATTTTCTAATTTCCCTGTCTCTTATA22.3529411764705883No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC22.3529411764705883No Hit
CTTATACACATCTCCGCGAGACGTCTTAGTACCTCGTATTCCGTCTTCTGC11.1764705882352942No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAAACAGAAA11.1764705882352942RNA PCR Primer, Index 36 (96% over 29bp)
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCG11.1764705882352942No Hit
GTCAAGCACAATGTATTTAGGCAGTATTATGTAAGTACTAGTTCTGTCTCT11.1764705882352942No Hit
GTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTCCGAGCCCA11.1764705882352942No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAGAATATGC11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
GTCCAGAACGTCGCCTCGCCGGCGCGGATGGCGTTCTGTCTCTTATACACA11.1764705882352942No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA11.1764705882352942No Hit
GATAGATAGGGGTGAGGAACTAATATAAACCCTGTCTCTTATACACATCTC11.1764705882352942No Hit
GCCGTGGGCGGCAATAACCTTCTCCAGCAAGGGGAGCAGGTCGTTCATCGA11.1764705882352942No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA11.1764705882352942No Hit
TTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAAT11.1764705882352942No Hit
TAAGAAGACAAAGGCTGGTGATGCCAAGAAGAAGTGAGATCCTGAAGAGAG11.1764705882352942No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAGTACCCCGC11.1764705882352942No Hit
ATGTTAATATGCATAACATGTACTCCTCACAAAAGAGTAGTAAAGCTAAAA11.1764705882352942No Hit
GATATGATTTTTGCAGATAATGGAGAGCAGTCAAGAGACCAACCAAAGTAG11.1764705882352942No Hit
GGTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACCTTTCT11.1764705882352942No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTGATACCACCCCGGC11.1764705882352942No Hit
TTCGTCTACATTTTTAACGTAATGTAAGTTTTCGAATTCGAATACATCTGT11.1764705882352942No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCCCCGTACTCTGCGTTGATACC11.1764705882352942No Hit
GCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT11.1764705882352942No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTC11.1764705882352942No Hit
GAGTTAAGCTGAGAAGGATGTATGTCACCTCAGGACCTTGTGATGATTGCG11.1764705882352942No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAGTCTGTCTCTTATAC11.1764705882352942No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGTATGCCGTC11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
GCAGACAGATACACACACACCCCTGTCTCTTATACACATCTCCGAGCCCAC11.1764705882352942No Hit
GGTTATCTACATTGATAACATTGAGAAAAGCTGAGTAAACACTGTACTGTC11.1764705882352942No Hit
GATGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAAAA11.1764705882352942Illumina PCR Primer Index 5 (96% over 28bp)
GGGCTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGT11.1764705882352942No Hit
CCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTTGTTGGTGGGGTAAC11.1764705882352942No Hit
GTGTAACCATTGTTCGATCTGAGAAGGTGAAGGATGAGATTGTTCTTGACG11.1764705882352942No Hit
CATGAGGGTCTCGTCCACACTCATCATGGCGCCAGCATTGTCAAACTCGCC11.1764705882352942No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTC11.1764705882352942No Hit
AGTTCAAACTCATTTCCCTTTTATTAAAGTCCAAGTTACCATTACATGGCT11.1764705882352942No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTACTGTCTCTTA11.1764705882352942No Hit
GTCCAGGGGGCCGCCTTCACCACCGGTATTCCTCCAGATCTGTCTCTTATA11.1764705882352942No Hit
AAAAAGTACTCTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGT11.1764705882352942No Hit
GATACCACACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC11.1764705882352942No Hit
TCTTTTACCACTTTATCAATGGTTGCATTATCTGTCTCTTATACACATCTC11.1764705882352942No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGCTGTCTCTTATACA11.1764705882352942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1764705882352942No Hit
CACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCC11.1764705882352942No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGT11.1764705882352942No Hit
TATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTAGAATCCCGC11.1764705882352942RNA PCR Primer, Index 39 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTACTCTGCGTTGATACCAGTGCTTCCCGTA11.1764705882352942No Hit
GTCTAATGTCTTTGTTTCCAAGATTTAGTTCTTGAAAAGGAATGATAGACT11.1764705882352942No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGC11.1764705882352942No Hit
GCAGAAGCTGTTGCCGAGCCACCAAAAGTTGAAGCAGTTGCTGAACGCACT11.1764705882352942No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAATGCCGTC11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGCCGCCTC11.1764705882352942No Hit
TTTATTGCTACATGGTGATTTTGAAAAAGATCCATTTCAAGTAAGATAAAG11.1764705882352942No Hit
CTTATACACATCTCCGAGCCCACGAGAGTCTTAGTATCTCGTATGCCGTCT11.1764705882352942No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATCATCGT11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAAAA11.1764705882352942Illumina PCR Primer Index 8 (96% over 27bp)
GTTTTAGGTCAGTCTGTAGGGATAATATCACTTGACTGCTATATTTCCCCT11.1764705882352942No Hit
CATTGGACAAAGGACATCTAAAAACAAACTGTATCCTTCTCAACAATTTCT11.1764705882352942No Hit
TATCAACTCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
CCTCCAGCATGCCTCACAGCACACCTTCGCAGGCTTACAGAACGCTCCCCT11.1764705882352942No Hit
GGCTACAATAGCAATAAAGATAGTTGCCAAGAGACTTAAGACCTGTCTCTT11.1764705882352942No Hit
CAACAACACATAGTGTCGCTGCCGCAGCTTCGGTGCATGGTTTAGCCCCGT11.1764705882352942No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCCAGAGTACTTTTTTTTT11.1764705882352942No Hit
GTTAGGTTCTACCTTGTTGTTTCATTGGAGCTTGTTTTAGTTGATTATTTC11.1764705882352942No Hit
GGATGATGGCAACCTCTTCAAGGTTTTCTTGTTAGTGTGGAAGTCAAGAGG11.1764705882352942No Hit
ACCGACGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACACTATCATT11.1764705882352942No Hit
CTTATACACATCTCCGAGCCAACGAGACGTCTTAGTATCTCGTATGCCGTC11.1764705882352942No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11.1764705882352942No Hit
GAGTACGGGGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGC11.1764705882352942No Hit
GTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTC11.1764705882352942No Hit
GCCGGTCCTTCAGGGCCGTCGCGGCCTCCGCGAACGACTTGGACACGCGGT11.1764705882352942No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACG11.1764705882352942No Hit
CACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAGCGTTC11.1764705882352942No Hit
TCAGTAACTTGAATCCACAGATATAAGCAGTATATAACCCTGTCTCTTATA11.1764705882352942No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACCAACGAA11.1764705882352942RNA PCR Primer, Index 27 (96% over 28bp)
CCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT50.045.018
AAACTCA50.045.06
ACATGGC50.045.044
TTACCAT50.045.036
ACCATTA50.045.038
TTTCCCT50.045.013
TTCAAAC50.045.03
TTACATG50.045.042
TTATTAA50.045.021
CATTTCC50.045.011
GTTCAAA50.045.02
TTTTATT50.045.019
TACATGG50.045.043
TTCCCTT50.045.014
ACTCATT50.045.08
ATTACAT50.045.041
AACTCAT50.045.07
CATTACA50.045.040
TCAAACT50.045.04
CCTTTTA50.045.017
Produced by FastQC (version 0.11.2)