FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_64.35100000044b3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_64.35100000044b3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22612
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC975843.15407748098355RNA PCR Primer, Index 39 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG4331.9149124358747567No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT4311.9060675747390767No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT490.21669909782416416RNA PCR Primer, Index 39 (100% over 21bp)
GCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT290.12825048646736248No Hit
CTTATACACATCTCCGAGCCACGAGACTGTTATACATCTCGTATGCCGTCT260.1149831947638422RNA PCR Primer, Index 39 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC10600.042.87735745
ATGCCGT10950.041.50684744
CTCCGAG11350.041.03524412
CCGAGCC11400.040.85526314
GAGCCCA11350.040.83700616
CGAGCCC11450.040.67685315
AGCCCAC11400.040.65789417
TCTCCGA11400.040.65789411
TCCGAGC11500.040.513
GCCCACG11450.040.48034718
CACGAGA11400.040.46052621
CCCACGA11400.040.46052619
ACGAGAC11400.040.46052622
CGAGACT11400.040.46052623
GAGACTG11350.040.44053324
TATGCCG11250.040.443
ACTGTTA11400.040.26315727
ATCTCGT11400.040.26315737
CATCTCG11400.040.26315736
TGTTATA11400.040.26315729