Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_64.35100000044b3b.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22612 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 9758 | 43.15407748098355 | RNA PCR Primer, Index 39 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG | 433 | 1.9149124358747567 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 431 | 1.9060675747390767 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT | 49 | 0.21669909782416416 | RNA PCR Primer, Index 39 (100% over 21bp) |
GCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 29 | 0.12825048646736248 | No Hit |
CTTATACACATCTCCGAGCCACGAGACTGTTATACATCTCGTATGCCGTCT | 26 | 0.1149831947638422 | RNA PCR Primer, Index 39 (100% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1060 | 0.0 | 42.877357 | 45 |
ATGCCGT | 1095 | 0.0 | 41.506847 | 44 |
CTCCGAG | 1135 | 0.0 | 41.035244 | 12 |
CCGAGCC | 1140 | 0.0 | 40.855263 | 14 |
GAGCCCA | 1135 | 0.0 | 40.837006 | 16 |
CGAGCCC | 1145 | 0.0 | 40.676853 | 15 |
AGCCCAC | 1140 | 0.0 | 40.657894 | 17 |
TCTCCGA | 1140 | 0.0 | 40.657894 | 11 |
TCCGAGC | 1150 | 0.0 | 40.5 | 13 |
GCCCACG | 1145 | 0.0 | 40.480347 | 18 |
CACGAGA | 1140 | 0.0 | 40.460526 | 21 |
CCCACGA | 1140 | 0.0 | 40.460526 | 19 |
ACGAGAC | 1140 | 0.0 | 40.460526 | 22 |
CGAGACT | 1140 | 0.0 | 40.460526 | 23 |
GAGACTG | 1135 | 0.0 | 40.440533 | 24 |
TATGCCG | 1125 | 0.0 | 40.4 | 43 |
ACTGTTA | 1140 | 0.0 | 40.263157 | 27 |
ATCTCGT | 1140 | 0.0 | 40.263157 | 37 |
CATCTCG | 1140 | 0.0 | 40.263157 | 36 |
TGTTATA | 1140 | 0.0 | 40.263157 | 29 |