FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_63.35100000044b11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_63.35100000044b11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133267
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC4304632.30056953334284RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT58684.403190587317191No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG49333.7015915417920415No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT2310.17333623477680143RNA PCR Primer, Index 39 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAAG206.988089E-445.0296673
TGCCGTC46200.044.07063345
ATGCCGT52950.038.45256444
CATCTCG57800.035.14816336
TATGCCG57950.035.13482743
CACGAGA58150.035.13005421
ATCTCGT57900.035.1263137
TATACAT57650.035.1225432
TCTCGTA58050.035.11305638
CGAGACT58050.035.11305623
GTTATAC57750.035.10067730
ACGAGAC58200.035.09987322
CCACGAG58200.035.09987320
GCCCACG58300.035.0782618
CCCACGA58250.035.06974419
GACTGTT57950.035.05718226
CTCGTAT58150.035.05267339
TCGTATG58150.035.05267340
AGCCCAC58350.035.048217
ATACATC57800.035.0313933
Produced by FastQC (version 0.11.2)