FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_62.35100000044af6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_62.35100000044af6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156030
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC7065645.28359930782542RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT107036.859578286227008No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG65074.170351855412421No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT4520.28968788053579436RNA PCR Primer, Index 39 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC73200.043.79873345
ATGCCGT84850.037.75860244
GCCGTCT2000.035.99794845
ATCTCGT92950.034.85546537
TCTCGTA93000.034.83672738
CATCTCG92800.034.81482736
CCGAGCC93600.034.8057114
CGAGCCC93550.034.80026215
TCCGAGC93650.034.78712513
GAGCCCA93400.034.78388616
TATGCCG92250.034.77850343
CTCGTAT92850.034.77184739
AGCCCAC93450.034.76527417
CGTATGC92750.034.76082241
GCCCACG93600.034.733618
TATACAT92900.034.72891632
GTATGCC92400.034.7220542
CCCACGA93650.034.71505419
CTCCGAG93800.034.70750812
TCGTATG92900.034.70469740
Produced by FastQC (version 0.11.2)