Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_61.35100000044ae9.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 358433 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC | 8505 | 2.3728283947069606 | RNA PCR Primer, Index 39 (95% over 21bp) |
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT | 1577 | 0.4399706500238539 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG | 941 | 0.2625316307371252 | No Hit |
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC | 522 | 0.14563391205608858 | No Hit |
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA | 494 | 0.1378221313327735 | No Hit |
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC | 481 | 0.13419523313980578 | No Hit |
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA | 433 | 0.12080360904269417 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 940 | 0.0 | 42.604797 | 45 |
ATGCCGT | 1100 | 0.0 | 36.612274 | 44 |
TATGCCG | 1185 | 0.0 | 33.98608 | 43 |
GTATGCC | 1205 | 0.0 | 33.421993 | 42 |
CATCTCG | 1215 | 0.0 | 32.776558 | 36 |
TGTTATA | 1205 | 0.0 | 32.675133 | 29 |
TATACAT | 1220 | 0.0 | 32.642227 | 32 |
CGTATGC | 1245 | 0.0 | 32.52891 | 41 |
ACTGTTA | 1205 | 0.0 | 32.48842 | 27 |
TCTCGTA | 1240 | 0.0 | 32.47863 | 38 |
CTCGTAT | 1245 | 0.0 | 32.348194 | 39 |
TCGTATG | 1255 | 0.0 | 32.269714 | 40 |
GTTATAC | 1230 | 0.0 | 32.011005 | 30 |
ATCTCGT | 1270 | 0.0 | 31.71142 | 37 |
CGAGACT | 1280 | 0.0 | 31.287899 | 23 |
CTGTTAT | 1260 | 0.0 | 31.248838 | 28 |
ATACATC | 1275 | 0.0 | 31.234133 | 33 |
TACATCT | 1290 | 0.0 | 31.045357 | 34 |
ACGAGAC | 1315 | 0.0 | 30.797335 | 22 |
CACGAGA | 1365 | 0.0 | 29.669224 | 21 |