FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_61.35100000044ae9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_61.35100000044ae9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358433
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC85052.3728283947069606RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT15770.4399706500238539No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG9410.2625316307371252No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC5220.14563391205608858No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA4940.1378221313327735No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC4810.13419523313980578No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA4330.12080360904269417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9400.042.60479745
ATGCCGT11000.036.61227444
TATGCCG11850.033.9860843
GTATGCC12050.033.42199342
CATCTCG12150.032.77655836
TGTTATA12050.032.67513329
TATACAT12200.032.64222732
CGTATGC12450.032.5289141
ACTGTTA12050.032.4884227
TCTCGTA12400.032.4786338
CTCGTAT12450.032.34819439
TCGTATG12550.032.26971440
GTTATAC12300.032.01100530
ATCTCGT12700.031.7114237
CGAGACT12800.031.28789923
CTGTTAT12600.031.24883828
ATACATC12750.031.23413333
TACATCT12900.031.04535734
ACGAGAC13150.030.79733522
CACGAGA13650.029.66922421