FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_6.351000000443fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_6.351000000443fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25152
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC1297951.6022582697201No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT5772.294052162849873RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG4981.9799618320610686No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT940.37372773536895676RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATTCGTATGCCGTCT260.10337150127226462No Hit
CTTATACACATCTCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT260.10337150127226462RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCCGAGCCACGAGACCCTAGAATATCTCGTATGCCGTCT260.10337150127226462RNA PCR Primer, Index 44 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCGTATGCCGTCTT260.10337150127226462No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14350.042.64808345
ATCTCCG15550.040.08038710
ATGCCGT15300.040.044
TCTCCGA15600.039.95192711
TCCGAGC15750.039.71428713
CCGAGCC15800.039.58860814
CTCCGAG15800.039.58860812
ACATCTC15800.039.4462058
CGAGCCC15800.039.44620515
AGCCCAC15650.039.3929717
GCCCACG15650.039.3929718
CATCTCC15800.039.30389
GAGCCCA15750.039.28571316
CCCACGA15750.039.28571319
CCACGAG15800.039.0189920
CACGAGA15800.039.0189921
ACGAGAC15800.039.0189922
GCCGTCT750.039.045
CGAGACC15800.038.87658323
TATCTCG15750.038.85714336
Produced by FastQC (version 0.11.2)