FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_59.35100000044aa6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_59.35100000044aa6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC63.7735849056603774RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCTTAATATCTCGTATGCCGTC10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGTAAGCCGTC10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTATACCCCGTTC10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGACAGCAGTC10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
CAATTAATCTATCTCTCTCTTTATCTCCCTCACACCTCAGCCTTCTGTGTT10.628930817610063No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAACCCACA10.628930817610063Illumina PCR Primer Index 8 (96% over 27bp)
ATCCCAAGGGCTTGGGGTCGGAATGTGAAAGGAGGGCCTGTGGCCCTGTCT10.628930817610063No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGATAATGATTGGCC10.628930817610063No Hit
TTTTTTTTTTTTTGCGTTGATACCCCCGTACTCTGCGTTGATACTGTCTCT10.628930817610063No Hit
GTTCTAATTATCACTCCTTTTTGCTAAGTTATTGACCATGCTAAAATTTTC10.628930817610063No Hit
GTACGGGGGTGCATGGAATAATGGAATAGGACCTCGGTTCTATTTTGTTGG10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACATATACAC10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
TTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATACACATC10.628930817610063No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGCTGTCTCTTATACACATCC10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAACATCTCGTATGCCGTC10.628930817610063No Hit
TATCAACGCAGAGTACGGGAGAGTACTTTCTCTGTCTCTTATACACATCTC10.628930817610063No Hit
TTCCTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTTTCCTT10.628930817610063No Hit
ACCTTGGGCAAGTCATTCAATTTCCCTAAGTCCTGTCTCTTATACACATCT10.628930817610063No Hit
ATATACTTCTATCAAGTGGAAAGGAAATTCCAGGCCCATGGAAACTTGGAT10.628930817610063No Hit
GATCAATCGTTCGTAATGTTAAAGGACCAGTAAGAGAGGGAGATATTCTTA10.628930817610063No Hit
GATGATGAACTTCTGCAAATGTCTCGTTCATGGGATATCGTAGGTATTGAT10.628930817610063No Hit
ATATTGAAGAGAGCAACTTGAATTCTTGCCTGCAAATCGGGCAATTCATTA10.628930817610063No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTCC10.628930817610063No Hit
CCTGTTTCCCATCGACTACGCCTTTCGGCCTCGCCTTAGGGCTGTCTCTTA10.628930817610063No Hit
TTGCTTCTCTACACGTAAAACTTGTTGGAAGACAGATTATCCTCCTAGAAG10.628930817610063No Hit
GTATCAACGCAGAGTACGGGTGGCTCCGATAGAACGCGGGCGGCAGGCCTA10.628930817610063No Hit
TCCCTACTTTGACAGCCTTGACAAATCTCAGTTCCTGTCACTTATACACAT10.628930817610063No Hit
GAATTACAAAAAGGCAAAATTGTGTTTTTCACAGAGATACAGTCCACTGGA10.628930817610063No Hit
TACCCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10.628930817610063No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATATAAATCTCGTATGCCGTC10.628930817610063No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCA10.628930817610063No Hit
GTATCAACGCAGAGTACGGGCAGCGGTAGCAACGCCTGTCTCATATACACC10.628930817610063No Hit
TTGTGTCATACCTTAAAGACTTGTTTTTCTGAGGTTATAAATACGTCCTGT10.628930817610063No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACAGGGGGAACTGAAA10.628930817610063No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCCAATAATC10.628930817610063Illumina PCR Primer Index 8 (96% over 27bp)
GCACAATAGAAAGGTATGGAGGACAATTACGAGGCAAACCAATAAGCATAA10.628930817610063No Hit
AGCTTTAATGTACGTGTGTGCATTGTTAAGACATTATCATCAGCAAAAATG10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACACCTTGTA10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
GCGGACCGGATTGCTCCGTTCCGCATCCGACCAGGACGCATCGCCGGCCCC10.628930817610063No Hit
GCGTAGGAGAAGCTATCGACAGCATCAGAACGGATTACGATCTGTCTCTTA10.628930817610063No Hit
CCCAGTGTGTTTCGACACACTACCATTACCCCGTCTCTTATACACATCTCC10.628930817610063No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCTGTCTCTTATACACATCTCC10.628930817610063No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGCTGTCTCTTATACACATCTCCGA10.628930817610063No Hit
GTGGTGCAAGTGCGTGGCAAAAGCGATACACGATATGTAATCTGTCACTTT10.628930817610063No Hit
TTTTTTACTATCTAGACACGAGTTTATCTCTGTCTCTTATACACATCTCCG10.628930817610063No Hit
ATATAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCACCAGCTTT10.628930817610063Illumina PCR Primer Index 12 (96% over 26bp)
TATACACATCAAGAGACTCCAACTTCCTCAACTACACACCCTTCATGCTGT10.628930817610063No Hit
GAATCCTTGTTAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCAGC10.628930817610063No Hit
TGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCCTGTCTCTTATACACA10.628930817610063No Hit
AGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC10.628930817610063No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACCT10.628930817610063No Hit
GCCTTGTGTTCCTCTTGAGTACCTCCTTCTAGGAACACTCCTGTCTCTTAT10.628930817610063No Hit
ACCCTTCATGTATTCATCTTTACCAAGAACTGCAAGGACTTGTTTATCAAC10.628930817610063No Hit
GCATAGTTGGTAAGACCAACAGTGAGTCCATACTGAGGAAGCTCATGTGCG10.628930817610063No Hit
GGGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGT10.628930817610063No Hit
CTTATACACATCTGCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC10.628930817610063No Hit
TCTTATACACATCTCCGAGCACGAGACAATGCGTTATCTCGTATGCCGTCT10.628930817610063No Hit
CTTTCCGACAGTGTGAAAATTTTGCCAAAGACCTTGAAATGTTTGCAAGAC10.628930817610063No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACCTGTCCATTATACACATCTAC10.628930817610063No Hit
TTCTTAGCCACAATAACAACTTAAAAGAGAAAGATCTGTCTCTTATACATC10.628930817610063No Hit
CTCCCATACCACGTGTCTTACCCATGTTTGTTGTTGGAGGAGAAACTGTCT10.628930817610063No Hit
CACGTCGAATCTTGGAGTGACGACAAGAGAGTTGAGAGAAGAGCCTGACTC10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTAAATATCTCGTATGCCGTC10.628930817610063No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATACAC10.628930817610063No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10.628930817610063No Hit
GTAGAGGAAAGCTTAATACTCTTTTTCCTGAAAGAATATCATCCCTGTCTC10.628930817610063No Hit
TTTCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC10.628930817610063No Hit
GTCGACACAAAGTCAATTGACAAGAGTGCTAAAGTTGGATTGGCTCTCGCA10.628930817610063No Hit
ATATTTCATGTTTAGCTTTCTCATGGAGAAAAAGAAACACAGGCATAAACC10.628930817610063No Hit
GTATTGGAGCTGGCATTGTTGACAAGTACAAGGACTGTCTCTTATACACAT10.628930817610063No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAACGGAGA10.628930817610063Illumina PCR Primer Index 8 (96% over 27bp)
GATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTCCCGTACTCTGCGTTG10.628930817610063No Hit
TGTCAGGGTTGTATCCGACCTTCTTCAAGTAGGACGCTGTCTCTTGTACAC10.628930817610063No Hit
CTACACTCAAACCACCTCACAGATGCTCTGCATTATTTAAGAAAGACAACA10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCTAGGCGAA10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTGATTTACATCTCGTATGCCGTC10.628930817610063No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTAAGCCGT10.628930817610063No Hit
GGTTTCATCCATCCGACATTGAAGTTGACTTACTGAAGAATGGAGAGAGAA10.628930817610063No Hit
GAGTCATTTTGGTCAAATATATAGGGGTAAACGAATCTAAAGGTTCACTGA10.628930817610063No Hit
AGCAGAGATTGATCTTCGCCGGAAAACAACTTGAAGATGGCAGAACCTGTC10.628930817610063No Hit
TAGCAAACCTTCCTTACCTCCAAAACGTAGTATCCGAGACCTGTCTCTTAT10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCTCTACACAT10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
ACATTGAAAATATGGTCCGGTCTTATGTTTAAAAGCCAAATTGCATCATAT10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGAATGCCGTC10.628930817610063No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGTCTCTTATA10.628930817610063Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGACAATGCGTTATCTCGTATGCCGTCTT10.628930817610063No Hit
GTACATGTGTAGAAGTGTTTATTACAGCATTGTTGTAAACACTTCATGTAT10.628930817610063No Hit
TCCCATGGTAGAAGATTTCTATGTGTCTCCTGTTTCACAGAAGCAAATGTC10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTTATTCATCTCGTATGCCGTC10.628930817610063RNA PCR Primer, Index 45 (95% over 21bp)
TGACTACACTGTGCTATACCAGAGAATTCCAGTAGAAAGACTGTCTCTTAT10.628930817610063No Hit
TGTCCCGCCCTACTCATCGAGCTCACAGCATGCTGTCTCTTATACACATCT10.628930817610063No Hit
ACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCAGGGAGGCCGAGCTTG10.628930817610063No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTCCTGTCTC10.628930817610063RNA PCR Primer, Index 36 (96% over 29bp)
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTTCGAATGGGGAAAC10.628930817610063No Hit
ACCTATTGACGAAAGAGGCGAAATTAAGACCGAACATTACCCGTCTCTTAT10.628930817610063No Hit
TCACAAAGCAGTGGAAAGGCTTGATGGAAGAGTTGTTGATGGCAGAGAAAT10.628930817610063No Hit
CACCGTATCCTTCATGAACTGGTCAATCAGATAGCCATGAGCCTGTCTCTT10.628930817610063No Hit
AGAAAAAGAGACAAGATAGAGAAGGGGAACAAGGAGAGGGAAATAGGGAAA10.628930817610063No Hit
CCTCTGGCCTCCGCATCTCCAGTCTGCTAATGGTGAATTGGATGAGGCCTG10.628930817610063No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTACTGTCTC10.628930817610063No Hit
TCTCTTCCTTTCTAGGAGCATTAGGATTACCCATCATATCCATCTCATTTT10.628930817610063No Hit
TTTTTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGG10.628930817610063No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTCGTTTTAACCCCCCGTACTCTGG10.628930817610063No Hit
CCAATGGATACTCGTTAAAGCTTTTAAAGTGTACTCATTCCAATTACAGGG10.628930817610063No Hit
GCCCTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTG10.628930817610063No Hit
CTCTTCTTCCGCCGCTTGCTCTGCTTACGAGCTTTACCTCCGTTTGCCTGA10.628930817610063No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCCG10.628930817610063No Hit
TGTTAAGTGTGATGTTTGGTTTGGTTTTGAACATTTCACATAATGGCTGTC10.628930817610063No Hit
TACCAATTCCCTGTTTATTATGGCTTTGGTGGAAGATATGCACTGTCTCTT10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACACAACCCA10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
ATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATC10.628930817610063No Hit
GCAAGAGAGATTGCACAGACCATAGCAAACTCGGCGAACAAGGTTTACTTG10.628930817610063No Hit
ACCACAAGCACCACCACAACTGCAACAACAACAACCTCCTACTTTTACCAT10.628930817610063No Hit
GTGTTGTCTGGCTTGACCCTGATCCGAACTTTGAGTTTCCAGAATCAAATC10.628930817610063No Hit
TGTCCCAGAGATTCTGGTATGTTGTGTCTTTGTTCTCCTTGGCTTCAAAGA10.628930817610063No Hit
CCTCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCT10.628930817610063No Hit
GCTATGAAGCATAACACACAGTTCTTTGTTGAATACTTCGCCTTGGATCTG10.628930817610063No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCT10.628930817610063No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCGTATGCCGTCT10.628930817610063No Hit
CATGCTGTGAGCTCGATGAGTAGGGTGGGACTGTCTCTTATACACATCTCC10.628930817610063No Hit
GTATCAACGCAGAGTACGGGTGACATAAGTAACGATAAAGCGGGTGAAAAG10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAATAACAGG10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
GTTGCCGAGAGATGACCAAGGATCCAATCATTCCCTTTGTTACGCGCCAGC10.628930817610063No Hit
CTCATATCCCGTATTTTTTCTTTAGCTCTTCTACCTGTCTCTTATACACAT10.628930817610063No Hit
TAACCGGACTATACCGGTAAGATATCCGGGTATCTAAACCTGTATCTTATA10.628930817610063No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATTTTT10.628930817610063No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTCAATGAC10.628930817610063No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGT10.628930817610063No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCCCCCACTC10.628930817610063RNA PCR Primer, Index 27 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTACCCCCCCC10.628930817610063No Hit
GGCAGGAACGTGATCCATGGTAGTGACTCTGTCGAGAGCTGTCTCTTATAC10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTTAACATCTCGTATGCCGTC10.628930817610063No Hit
TCTAAGAAGATCTTAAGAGATAACATGAAGGCTTATTTAAACAGCTGTCTC10.628930817610063No Hit
ACCAATAACATTAATCTCCCCAAGTCGTTGCATGTATAGACTGTCTCTTAT10.628930817610063No Hit
GTATCAACGCAGAGTACGGGGCAGTAGTATCGACGCAGAATAAATAATATA10.628930817610063No Hit
CAGAAAAGACTACTAAGAAAACTTCATCAAAGAAGACGCCAGTGGCTAAGG10.628930817610063No Hit
ATGCTAACTGTCCATATTCACATAGAGACACAGTGTACTCCTGTCTCTTAT10.628930817610063No Hit
TTCTTAATGTAAGCACTGAAGTGAGTTTGTAACTTCTCTGGCTCTGTCTCT10.628930817610063No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATACGAACCGTTATAACCGG10.628930817610063No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT10.628930817610063No Hit
TTCTTGCGTTGATACCACCCGTACTCTGCCTGTCCCTTATACACATCTCCC10.628930817610063No Hit
GGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACC10.628930817610063No Hit
GGTGACGATTCATGAACACTAAAAGGCATATCTTGAGTCATTTTCATACCT10.628930817610063No Hit
GAGGAATTAGTAGATGTTTCCTAAACTTACTTCCACACATAGGATTCTTTA10.628930817610063No Hit
AGTGTGTTTCGACACACTATCATTAACCTGTCTCTTATACACATCTCCGAG10.628930817610063No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC10.628930817610063No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10.628930817610063No Hit
GCGTGTATGTTTGTGCTTGTGTGTCTTTTTTTCTGAACTAAGAGTAAATTG10.628930817610063No Hit
CTGTTGAATCAAAATCTCTAGAGTTTAAATTAAGATTTCCACCAGCTTTAC10.628930817610063No Hit
CAAGAAACAGTTCTCTCAATACATAAAGAACAGCGTAACTCCAGACATGAT10.628930817610063No Hit
GTATGAACGCAGAGTACGGGGATATAGTCTGTCTCTTATACACATCTCCGA10.628930817610063No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers