FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_58.35100000044a8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_58.35100000044a8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295145
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC4396714.89674566738383RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT183626.221348828541903No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG30891.046604211489268No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC30801.043554862864016No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA16860.5712446424638737No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC15460.5238103305155093No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA14590.49433329380474No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC12440.421487743312609No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA11090.37574751393382916No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA9100.30832302766436837No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG7020.23784919276965558No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG6750.2287011468938996No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA6590.22328008267122942No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC6160.20871097257280322No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC5750.19481949550221078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA5730.194141862474377No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC5500.18634908265428857No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5170.17516813769503126No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT4980.16873062393061036RNA PCR Primer, Index 39 (100% over 21bp)
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA4920.16669772484710904No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4820.16330955970794017No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG4430.1500957156651815No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT4320.14636873401209574No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC4210.14264175235900997No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG4170.14128648630334242No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC4100.1389147707059242No Hit
AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC3910.13247725694150334No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA3860.1307831743719189No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT3630.12299039455183046No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC3340.11316471564824071No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA3260.1104541835369056No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3140.10638838536990293No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC3080.10435548628640161No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT3070.1040166697724847No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC46300.043.49204645
CTAGTAG250.002101427436.0109833
ATAACGC250.002101427436.0109833
TAAGACA250.002103176736.0048834
GAACTCG250.002104926935.9987815
TATGCGT250.002104926935.9987810
CGAGGAA250.002104926935.9987820
ACTCGAG250.002104926935.9987817
GCCGTCT1500.034.4988345
CGTCTTC352.8158925E-432.1417745
ATGCCGT66450.030.30371544
GGATGTT300.00513346830.0091531
GGCTAAT300.00513346830.0091531
AGATCTT300.005137725330.0040665
GAATCCC453.482954E-529.99898524
TAGCAGA300.00514198529.99898322
GTTCGAA300.00514198529.99898311
TCGAGGA300.00514198529.99898319
AGAGGGT300.00514198529.9989838
TTCGAAC300.00514198529.99898312
Produced by FastQC (version 0.11.2)