FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_57.35100000044a62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_57.35100000044a62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170684
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC2640315.468936748611469RNA PCR Primer, Index 39 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT114786.724707646879614No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG26741.5666377633521595No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC26281.5396873755009257No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA15000.8788169951489302No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC12080.7077406200932718No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA10700.6268894565395702No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA8370.49037988329310306No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC5700.33395045815659347No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA5360.31403060626655105No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5120.2999695343441682No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT4700.27536265847999813RNA PCR Primer, Index 39 (100% over 21bp)
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG4680.2741909024864662No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG4360.2554428065899557No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC4250.2489981486255302No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA4120.24138173466757284No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG3950.2314218087225516No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA3580.20974432284221134No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC3540.2074008108551475No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC3180.18630920297157322No Hit
AAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCC3170.18572332497480726No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT2830.16580347308476484No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT2480.14529774319795646No Hit
GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG2470.1447118652011905No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC2410.14119659722059477No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC2290.13416606125940334No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA2260.13240842726910548No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC2200.12889315928850978No Hit
AAAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2060.12069086733378642No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC2020.11834735534672261No Hit
GTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1940.11366033137259496No Hit
AAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACGAG1840.10780155140493544No Hit
GGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGCCC1800.10545803941787162No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT1760.1031145274308078No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1740.1019427714372759No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG207.0076145E-445.011432
TGCCGTC29200.043.91951845
TCTTAGG250.00209947536.0091443
TAACGCA250.00209947536.0091444
TCTAAGC250.00209947536.0091443
ATAACGC250.00209947536.0091443
CACATTG250.002102498235.99859625
ACTATGC352.811033E-432.14168
ATGCCGT40650.031.54858244
GCCGTCT602.399429E-729.99882945
TATGCCG43900.029.21298243
ATCTCGT44000.029.09545337
GTATGCC44100.029.08049842
CTCGTAT44050.029.0624339
CGTATGC44150.029.04756241
TCTCGTA44100.029.02947838
CGAGACT44200.029.01470423
CATCTCG44050.029.01135336
TCGTATG44250.028.98191840
GAGACTG44000.028.9420524
Produced by FastQC (version 0.11.2)