FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_56.35100000044a49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_56.35100000044a49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151114
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC2820018.661407943671666No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT80425.321810024220125No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG12520.8285135725346428No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT5170.3421258123006472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC29300.043.08020845
GCCGTCT1250.036.045
AACAGCC250.002101334535.99999616
ATGCCGT37300.033.8404844
CGTATGC38850.032.54826441
TATGCCG38850.032.54826443
GCCCACG38900.032.50642818
CCCACGA39000.032.48076619
ACGAGAC39100.032.45524622
TCTCGTA38700.032.4418638
ATCTCGT38650.032.42561337
GATCTCG38450.032.41872436
CCACGAG39150.032.41379520
GAGCCCA38900.032.39074716
AGCCCAC39050.032.3815617
GTATGCC39050.032.3815642
CCGAGCC38850.032.3745214
CGAGACT38850.032.3745223
CACGAGA39300.032.3473321
ACCGCTG38400.032.34375430
Produced by FastQC (version 0.11.2)