FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_55.35100000044a2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_55.35100000044a2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177289
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC4958827.970150432344926No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT1977811.155796467913971No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG19231.0846696636565156No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT9820.5538978729644818No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG2960.16695903299133052No Hit
ATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCTTC1800.1015291416839172TruSeq Adapter, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCC207.024287E-444.99069215
CGACTGA207.024287E-444.99069213
TGCCGTC55250.042.9956345
GCCGTCT2500.032.393345
GTGTAAT352.8002844E-432.163431
AGTATTA352.8095808E-432.14527534
TAACATG352.8142385E-432.1362113
ATGCCGT75200.031.61912244
CGTATGC77300.030.87653541
CCCACGA77400.030.87441319
ACGAGAC77500.030.86361322
TATGCCG77050.030.85993643
CCACGAG77550.030.84371420
CGAGACT77200.030.83781223
TCTCGTA77350.030.83619338
GCCCACG77500.030.83457818
CACGAGA77600.030.8238421
CGAGCCC77800.030.8226715
AGCCCAC77700.030.80443817
ACCGCTG77000.030.8010230
Produced by FastQC (version 0.11.2)