FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_54.35100000044a05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_54.35100000044a05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24872
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC423517.027179157285303No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT1780.715664200707623No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG1370.5508201994210358No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA206.9098733E-444.9879316
TGCCGTC4350.042.91951845
ATGCCGT4500.041.4888744
ACCGCTG4650.040.6342630
TACCGCT4650.040.6342629
CACGAGA4600.040.58693321
ACGAGAC4600.040.58693322
CGAGACT4600.040.58693323
CTCGTAT4600.040.58693339
GAGACTA4600.040.58693324
CCGCTGA4700.040.20197731
CTACCGC4700.040.20197728
ACTACCG4700.040.20197727
GACTACC4700.040.20197726
GTATGCC4650.040.15051742
CCACGAG4650.040.15051720
TATGCCG4650.040.15051743
CGTATGC4650.040.15051741
GCTGATC4650.040.15051733
TCGTATG4650.040.15051740
Produced by FastQC (version 0.11.2)