FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_53.351000000449ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_53.351000000449ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79307
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC3160.3984515868712724No Hit
GGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCG1220.15383257467814945No Hit
CTTAAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTA1060.13365781078593314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTG206.994067E-444.9993711
TACGAGT206.994067E-444.999374
ACGAGTC206.994067E-444.999375
GTCGGGT206.994067E-444.999379
CCACGAG750.038.9994520
CGTATGC800.036.5619941
TATGCCG800.036.5619943
CCCACGA800.036.5619919
GTATGCC800.036.5619942
ACTACCG800.036.5619927
GTACCGG250.002088985236.022211
CGAGTCG250.002095469735.9994966
GAATGCA250.002095469735.99949623
TGTGATT250.002095469735.99949623
AGTCGGG250.002095469735.9994968
GCCCACG850.034.41128518
CTACCGC850.034.41128528
CGAGACT850.034.41128523
ATGCCGT850.034.41128544
TACCGCT850.034.41128529
Produced by FastQC (version 0.11.2)