FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_51.351000000449a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_51.351000000449a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC42.4390243902439024No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACT21.2195121951219512No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC21.2195121951219512No Hit
GCGTTGAGCAAGGAAGAGAATTCTGCTTGAGAGTATCAATTTTCTTATGTT21.2195121951219512No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAC10.6097560975609756No Hit
CGGGAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT10.6097560975609756No Hit
GAAAAAGAAGAAGATTGTTGAAGGAGATCATGTGAAAACAGTGGAAGAAGA10.6097560975609756No Hit
CCAGCTTCTCAGACGAGAGCCAAGGTTCCTTTTCAACTAGCTCATTTAGAT10.6097560975609756No Hit
TATTTGACATGTACAACAGATCCACTTACGCATCCTTGTTTACTTGTTCAA10.6097560975609756No Hit
GATGGAATCATTGCGAATTACATAGAAGCTACCAGCCTAAGCCAAACCCAT10.6097560975609756No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAA10.6097560975609756No Hit
TCTCTGGATTTATCTTCAAAACAGGAAAAGGTTTTGGCTGTCTCTTATACA10.6097560975609756No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGATAGATC10.6097560975609756RNA PCR Primer, Index 27 (96% over 28bp)
GTCCAGAGTCTGATGAACACATATTACCTCATATTTCTCTGCTTGTTTGAT10.6097560975609756No Hit
GCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGTATTCCTCCTCTA10.6097560975609756No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTACTGTC10.6097560975609756No Hit
GTATGTTGTCGATATTGACAAAAGCATAGACATAACGAAACTCACTCCATC10.6097560975609756No Hit
AGAAAGAACGAAGGATTGATGATAGTACTTTGAATCTGTCTCTTTTACACA10.6097560975609756No Hit
GTGTTGTTATAATGAAGATAAGGTACACACTGAAACCACTGTCAGATTAAG10.6097560975609756No Hit
GAAAGAAAGAAATGAATTGAATTTCAATAAGGGTTTCCTGGATTGTGGGTA10.6097560975609756No Hit
AAACAAACATATGAAGCTCCTTAGCATCATTACTATTTGATCTGTCTCTTA10.6097560975609756No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACAA10.6097560975609756No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGTTCACTCCT10.6097560975609756Illumina PCR Primer Index 8 (96% over 27bp)
ATGATCTTGTCACCTTCCATTACTTTTTCCCATTCAAATCTTTCAGCGATA10.6097560975609756No Hit
GAATAGATATTACACCATATTGTCTCTATGTTCATCCTGTCTCTTATACAC10.6097560975609756No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCCCC10.6097560975609756No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAATGGAAAAAC10.6097560975609756RNA PCR Primer, Index 36 (96% over 29bp)
CCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCATATGCTGGCTCTT10.6097560975609756No Hit
AAGTACGTATGTTCTACAGTTGACCAAACATGGAATTCCTAAAACCACATA10.6097560975609756No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC10.6097560975609756No Hit
ACTCATCGTACTTGTTGTTGTTTTTAGTGTTGTAATGTTTTAATGTTTCTT10.6097560975609756No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTACCCCCCCCACTCCTCG10.6097560975609756No Hit
GTATATAGCAGAGAAGTATAGATCACAAGGACCTGATCTTTTGGGGAAGAC10.6097560975609756No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGCTGTCTCTTA10.6097560975609756No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA10.6097560975609756No Hit
GAAAAGATAAGAAAATTAGCAATGCGAAACAATGCCATTGTGTAAAATCTG10.6097560975609756No Hit
ATATTATCTGATTGCGTATTCACATTTGGTGTCCACATGGGATGAATATTG10.6097560975609756No Hit
GTGTAGTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAA10.6097560975609756No Hit
GTGTTACAACTGGACGACACGGTGAATAAGAAGTCTTAACACTGTCTCTTA10.6097560975609756No Hit
CTTCAGAACCTGAGCTTGGTAAAGAACCATCTGTTCTTACCCTGTCTCTTA10.6097560975609756No Hit
ACAAAAAACAAAAAACAACATAAGTCCCACTCCCACATCATTATTACTGTC10.6097560975609756No Hit
AGATGAGACAGATACTAAGTCATACAATGACAAGTTTGTATCTGTCTCTTA10.6097560975609756No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACCTAATTGTTG10.6097560975609756RNA PCR Primer, Index 36 (96% over 29bp)
GTTTGAAGCAGCTAGTGTTGTTGAAGATGTTAAGAAGAATCCAGATTCTGC10.6097560975609756No Hit
CATTTATTCACCACCACACATAAGAGCACATTTAAGGTGAAAAAGACTCTG10.6097560975609756No Hit
AGTGTTTATGTTTCTCTATGAGAATCATGTAATGTCTCTGATTTGATTCGG10.6097560975609756No Hit
GGGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCT10.6097560975609756No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAGAGTACTTTTTCTGTCTCT10.6097560975609756No Hit
CAACAGGCAGTGGAAGCAGTTTCAGAGAACTTTTTGCATGCTTATGGTCTG10.6097560975609756No Hit
GTTATTGACCTCCAGAAGCAGTAGTGACCCTCTCTCTTTCTTCACTTCTTT10.6097560975609756No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAAT10.6097560975609756No Hit
GATGATGCACTTCTTTACCCCTTTGGTTTTTATATTCGTACAATGACGTGG10.6097560975609756No Hit
GAATATAACATCCAGAGATATCGTAGTACTCAATCATATCTTGTTTACAAA10.6097560975609756No Hit
GCTTAGGGACGTACTCTGCGTTGATACCACTGCTTAGGGACGTACTCTGCG10.6097560975609756No Hit
GTATATATGTGTCAAACTCAATAAATGCATATGGCCTTGTTACTGAGTCTG10.6097560975609756No Hit
GTTTCGACACACTATCATTAACTGAATCCATAGGCTGTCTCTTATACACAT10.6097560975609756No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTCATGTTTATACCACCCGTACACT10.6097560975609756No Hit
CTTTAATCTTTTCTGCTATACCTTTTTTCTCATGGTGAACGACATTAGTGT10.6097560975609756No Hit
GACTTATTCTCTTGATGAAAGTCATTGGACGCTAGTATAATGGGTTGGTTA10.6097560975609756No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGC10.6097560975609756No Hit
GGATTATCTGTATCACAGGTTTCGAGTTTTGAGATATGGGTTTTGTCTTTA10.6097560975609756No Hit
GATAAAAAGACAAACTAAACTTGTCGTGATTCTATATCTGTCTCTTATACA10.6097560975609756No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT10.6097560975609756No Hit
TTACACAATCACACCATTACCATACATAGGAACTGTCTCTTATACACATCT10.6097560975609756No Hit
CCGTAATAATGATGTCTTTGAACTATTGTTCCGCGACATTTACGTTCCGAA10.6097560975609756No Hit
GGTATCAACGCAGAGTACGGGGTCTGTCTCTTATACACATCTCCGAGCCCA10.6097560975609756No Hit
GTCACAACTTGTAGAGCCAAAGATACATTTTCACTCATTGTTCCATACCCA10.6097560975609756No Hit
GGGAAAGCTCCCGAGAGAGCCGTTCCCAGTCCGTCCCCCGGCCGGCACGCG10.6097560975609756No Hit
CGTGATTCTGATTGCTTATGCTCACAGCGTCAGTTGAGATGCTCCTGTCTA10.6097560975609756No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT10.6097560975609756No Hit
GAACACATACTATGTACCAGATACTACATTTACCTCTGTTTCTGTCTCTTA10.6097560975609756No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCCTATCTCGTATGCCGTC10.6097560975609756No Hit
CTTATCAGACAATCCAATTATTTGTCCTCCATATCGAACAAGGTATGGAGT10.6097560975609756No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC10.6097560975609756No Hit
GTATTAGCATCCTCCACACTTCCATTTCCAACACTAGCATTAGATCTAGCC10.6097560975609756No Hit
AATGTACTTAAGATGCTCATCTTCAGGAGGAATACATTTCTTGATAACATC10.6097560975609756No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCACAACCCTA10.6097560975609756Illumina PCR Primer Index 8 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTACTGCTTATCTCGTATGCCGTC10.6097560975609756No Hit
CTTATACACATCTGCCCACGAGACAATGCGTTATCTCGTATGCCGTCTTCT10.6097560975609756RNA PCR Primer, Index 31 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACCGCTTATCTCGTATGCCGTC10.6097560975609756No Hit
AGCCAGATTTGTTCGAAAACATGGATGATAAGATCATGATACTCCTTTGGA10.6097560975609756No Hit
GTACTTTTTTTTGTTTTTTTTTTTTTTTTTTATTTTTCCCTTGATACCCCC10.6097560975609756No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGC10.6097560975609756No Hit
GTGTTATGAGTATTCTTTCTAAATGCTGTCCATTTTTAGCCCTAATGTACC10.6097560975609756No Hit
GTAAAGATTTGATCTTTTTCTCTGGTTGTGATCTATAAGATAGCTCCTGTC10.6097560975609756No Hit
TAATCAATCACTTAAACATAAGCGTTCAAACATAGCAGGAGGACGCTATTT10.6097560975609756No Hit
ACTTTCCCGCGAAAGGAATTATATTAGTCGGAGAAGGCTCGAAACGTAGTG10.6097560975609756No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATCTCCGAG10.6097560975609756RNA PCR Primer, Index 27 (96% over 28bp)
ATTTTAGACCATAATAATTTCATCAACAGTTACAGTTTGACCCACAGCTGC10.6097560975609756No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC10.6097560975609756No Hit
ACAGTGTTTTCACCATTAATATTTATGATCTTGGTCTTTCCTTCTTGCCTT10.6097560975609756No Hit
TTCTAGAATACCAGGGTAAACAAGGAGACGTTATTCAAAGATGAATGAGAA10.6097560975609756No Hit
GTTTTTGATTTCTCAAGAGGAGAAATGACTCAGCAGAAGATTAAGAGCCTA10.6097560975609756No Hit
GGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGT10.6097560975609756No Hit
CCGTTAATTGTTACGATTTTAGCCGTCGTCGACGAAGAAACGTACTCGTTT10.6097560975609756No Hit
CTTTAAATCCAAACAAGTAGACACAATTACAATCGTAGTCAAACTTCATCT10.6097560975609756No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCCCCTTTTCCCCCC10.6097560975609756No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT10.6097560975609756No Hit
AAATAATAGTGGCAAAACATAACACTCCTGGACTGCGGGAAATAAAATTGA10.6097560975609756No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTCTCCTACCT10.6097560975609756RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTACGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC10.6097560975609756No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTCATT10.6097560975609756No Hit
ATCATAGGCATTATTTATGAAGCCTTAAGATCCAGAAGTGTTGTTACTACC10.6097560975609756No Hit
TACCTAATCACGAACTGTTACATCAAATCTAAAGCAGAACCAGAACAAAAT10.6097560975609756No Hit
GTGTGTAACAACTCACCTGCCGAATCAACTAGCCCCGAAAATGGATGGCGC10.6097560975609756No Hit
CATTATACATTACAGAGTGTAGATGATCAGTTTGGAATCTGAATCAAAAAC10.6097560975609756No Hit
ATCCATATAAGTACTGTGAAAGTGAAAGCTTCTTATGTAACTGTCTCTTAT10.6097560975609756No Hit
AAACAGTACAAGTGCAAGTCTATCTTCTTTTCCTCGATTTGAAGCCTCTCT10.6097560975609756No Hit
CCATAATAATGTGTGAGTAGTTCCCAGATAAGGAAATTAGGGTTCCTATAG10.6097560975609756No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT10.6097560975609756No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAACTGTCTCTTATA10.6097560975609756No Hit
AGACTATTGTGTTCAAGTCTGGTATGGCTAAGTCTATCCATCACTCTCGTG10.6097560975609756No Hit
GTCTATGAGTTGAGCTGTCTCTATATTTCTTAGCCAAACCATAGTCTATGA10.6097560975609756No Hit
CAGGAGAATTGGTACCGTAGTAAGTAAGGGTTAGAGAGAGAAAGAATGTCA10.6097560975609756No Hit
TATCAACTCTCTTAAGCTTTGACTTTTCCCAGCTTTGTATTTTCCTATGTA10.6097560975609756No Hit
AATACATACTAAGGGTTTCTTATATGCTCAACACATGAGCGAAACCCTATA10.6097560975609756No Hit
TCCCCGGCGGGCGCTGCGGCCAGGGAACCCGTCGAGGTGAAGCTGACGCTC10.6097560975609756No Hit
TTCAATGTACAGAGATTATTCAATCTCCCTAGAAACATACCTGTCTCTTAT10.6097560975609756No Hit
GAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCG10.6097560975609756No Hit
GTTCACCTCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACA10.6097560975609756No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTT10.6097560975609756No Hit
TCGTTATCGTTATCTACTGCTTCTGATTTCTGTTTGTTCTCTTCCTGTCTC10.6097560975609756No Hit
GGTAAAGACTGCTTTATTGGTGGCAGTTACTACAAGTCAATAAATATTGAT10.6097560975609756No Hit
ATGTTAGTCTGCATAATAATTTTGTTGTGTGAATATCTGTCTCTTATACAC10.6097560975609756No Hit
TTTCTGAATATTAATACGTTTTGTACAAGTTGCTCAAGATTGTACACCATA10.6097560975609756No Hit
CCCACCAAGAAGCCAGCTCAAATGCTGAAACCTGTCTCTTATACACATCTC10.6097560975609756No Hit
TGTAAGCATCTTTTATCGTGAATTCCGTGACTTGAATAGAACTAGCAAGTG10.6097560975609756No Hit
ATCAACTACCACTGTATCGTGTACCTTAATATCTGTCTCTTATACACATCT10.6097560975609756No Hit
TGGTTGGAATACCTGATATACACAGTAAGAGATCAACATAAGAACCTGTCT10.6097560975609756No Hit
GATAAAACTTTGCAGAGATATCTTAATGACCAAACCATAAAAACACACACA10.6097560975609756No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATCGGTTACTGA10.6097560975609756No Hit
GTTCAAGTAAGCTTTGTGGACATCTATTTCTCTGTCATCCAATGGTTTACC10.6097560975609756No Hit
CCACCCCGTACTCTGCGTTGATAGCACTGCTTCCCGTACTCTGCGTTGATA10.6097560975609756No Hit
GATTGGTTTAGTCAATAAATGGACGTGATTGGATCTAAAACTGTCTCTTAT10.6097560975609756No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATATACAACA10.6097560975609756Illumina PCR Primer Index 8 (96% over 27bp)
GTATAGTGCAAGTATTACGGAGGTTTGAATATAGAGTGAGTAGATAGATGC10.6097560975609756No Hit
GATTACATGCGCTACTTAATGGAAGAAGATGAAGATACTTACAAGAAACAG10.6097560975609756No Hit
CTCTAACACTGATCTAGTGAAGACTAAGGTAAGGCTGTCTCTTATACACAT10.6097560975609756No Hit
TCTTTCAGCAGTTATCTGTATCTCTCCCTTTTCCCACTTCCCACTGGCAAT10.6097560975609756No Hit
ACCCTGGGATGTCCATATTGACGCCGAGCTTAGCGCGGACGCCCGATCGAC10.6097560975609756No Hit
GCTGAAATATCCCGTGAACTGGTTTCATTTCATATATGCTTTGATGATGAT10.6097560975609756No Hit
ATCTTTGAACATGCCATTCACATCACGAATCTGGTCTTCAATTTCTCGAAT10.6097560975609756No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTC10.6097560975609756No Hit
AATTAATCTTTGTATACGAGTCACTAAAAGCTGTCTCTTATACACATCTCC10.6097560975609756No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCCTTATCTCGTATGCCGTC10.6097560975609756No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA10.6097560975609756RNA PCR Primer, Index 27 (96% over 28bp)
GTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATCTGTCTC10.6097560975609756No Hit
GAACTGAATCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC10.6097560975609756No Hit
CTCCACACTTTACTCTTTGGAAGTCACTATATGTAGCCTGTCTCTTATACA10.6097560975609756No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCCCTTATCTCGTATGCCGTC10.6097560975609756No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCCGCTTATCTCGTATGCCGT10.6097560975609756No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAACCAGAACC10.6097560975609756RNA PCR Primer, Index 27 (96% over 28bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGC10.6097560975609756No Hit
ATCAACGCAGAGTACGGGATACCAGCTGGCTGCAACTGTTTATTCAAAACA10.6097560975609756No Hit
ACCTAGCAGAGGAATACAGTGAGAAAGGTGAAAAGCTTATCTCCCAAGACC10.6097560975609756No Hit
TATCAACGCAGAGTACGGGAAGCAGTAGTATCCTGTCTCTTATACACATCT10.6097560975609756No Hit
TTCAAAAGTAGTAGAAGCTTTCCAAAGAGACAAGATAGGACCTGTCTCTTA10.6097560975609756No Hit
GATCATAAGGAGTGGTTTGGGAAACCTTTGAAAGATTCATTTTACTACAAA10.6097560975609756No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers