FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_50.3510000004498d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_50.3510000004498d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158305
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC2360114.908562584883612No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGT82645.220302580461767No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCG11520.7277091690091911No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT3040.1920343640440921No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC2580.16297653264268344No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC2020.1276017813714033No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA1880.11875809355358327No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1630.10296579387890466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25900.043.6085145
GCCGTCT800.042.1860245
ATGCCGT33750.033.46549244
TATGCCG35300.031.99604443
CGTATGC35500.031.87916241
ATCTCGT35300.031.8685737
CTCGTAT35400.031.84210439
TCGTATG35550.031.83432440
GATCTCG35150.031.8125436
GTATGCC35450.031.79719242
TGATCTC35200.031.76735335
CGCTGAT35350.031.75984832
CCGCTGA35350.031.75984831
TCTCGTA35450.031.73372538
CTGATCT35200.031.70343434
ACGAGAC35700.031.70056722
GCTGATC35250.031.65846433
CACGAGA35750.031.6562321
ACCGCTG35400.031.65143230
TACCGCT35400.031.65143229