FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_5.351000000443d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_5.351000000443d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56205
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC1314023.378702962369896No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT6111.0870918957388132RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG6031.0728582866293035No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT890.15834890134329688RNA PCR Primer, Index 44 (95% over 23bp)
GCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT710.12632328084689975No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG590.104972867182635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14600.043.6078445
GAGACCC15400.041.6346724
ATGCCGT15300.041.61271344
CCCACGA15600.041.53353519
ACGAGAC15600.041.53353522
CGAGACC15450.041.4999323
AGCCCAC15750.041.4236617
CACGAGA15550.041.37772821
GACCCTA15500.041.36606226
GCCCACG15750.041.2808218
CCACGAG15650.041.25708420
ACCCTAG15550.041.2330527
CTAGAAT15550.041.2330530
CCTAGAA15500.041.22091729
CCCTAGA15500.041.22091728
TAGAATA15500.041.22091731
AGACCCT15600.041.1008925
GAATATC15600.040.9566833
CTCGTAT15600.040.9566839
AGAATAT15650.040.8258332