Basic Statistics
Measure | Value |
---|---|
Filename | H23LNBCXX_l02n01_bir_0113_pb_5.351000000443d3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 56205 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC | 13140 | 23.378702962369896 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 611 | 1.0870918957388132 | RNA PCR Primer, Index 44 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG | 603 | 1.0728582866293035 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT | 89 | 0.15834890134329688 | RNA PCR Primer, Index 44 (95% over 23bp) |
GCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT | 71 | 0.12632328084689975 | No Hit |
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG | 59 | 0.104972867182635 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 1460 | 0.0 | 43.60784 | 45 |
GAGACCC | 1540 | 0.0 | 41.63467 | 24 |
ATGCCGT | 1530 | 0.0 | 41.612713 | 44 |
CCCACGA | 1560 | 0.0 | 41.533535 | 19 |
ACGAGAC | 1560 | 0.0 | 41.533535 | 22 |
CGAGACC | 1545 | 0.0 | 41.49993 | 23 |
AGCCCAC | 1575 | 0.0 | 41.42366 | 17 |
CACGAGA | 1555 | 0.0 | 41.377728 | 21 |
GACCCTA | 1550 | 0.0 | 41.366062 | 26 |
GCCCACG | 1575 | 0.0 | 41.28082 | 18 |
CCACGAG | 1565 | 0.0 | 41.257084 | 20 |
ACCCTAG | 1555 | 0.0 | 41.23305 | 27 |
CTAGAAT | 1555 | 0.0 | 41.23305 | 30 |
CCTAGAA | 1550 | 0.0 | 41.220917 | 29 |
CCCTAGA | 1550 | 0.0 | 41.220917 | 28 |
TAGAATA | 1550 | 0.0 | 41.220917 | 31 |
AGACCCT | 1560 | 0.0 | 41.10089 | 25 |
GAATATC | 1560 | 0.0 | 40.95668 | 33 |
CTCGTAT | 1560 | 0.0 | 40.95668 | 39 |
AGAATAT | 1565 | 0.0 | 40.82583 | 32 |