FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_48.3510000004494a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_48.3510000004494a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146681
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC3195121.78264396888486RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT86515.897832711803164No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG14200.968087209659056No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT6710.45745529414170893No Hit
ATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTTC2030.13839556588787916RNA PCR Primer, Index 20 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC35600.043.03746845
TCACTAG250.002091376336.034131
ATAACGA250.002098396736.009555
GTGCAGG250.002101913835.99727618
ATGCCGT45200.033.84699244
AGCCCAC47500.032.6343717
TATGCCG46950.032.6333143
CACGAGA47300.032.6296621
CCCACGA47300.032.6296619
CCGAGCC47300.032.6296614
CCACGAG47300.032.6296620
GCCCACG47450.032.6213418
CGAGCCC47450.032.6213415
GTATGCC47000.032.59859542
TCCGAGC47350.032.59520713
ACGAGAC47350.032.59520722
CGTATGC47300.032.58209641
ATCTCGT47300.032.53453437
CTCGTAT47300.032.53453439
GAGCCCA47650.032.5316416
Produced by FastQC (version 0.11.2)