FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_47.35100000044920.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_47.35100000044920.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73556
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC3500147.58415357006906RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT836011.365490238729675No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG15652.1276306487574095No Hit
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT2150.29229430637881343No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTTGACATCTCGTATGCCGTC1110.15090543259557343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37750.044.2204945
GCCGTCT659.094947E-1238.07289545
TACGGGG250.00209542635.9961914
ATGCCGT46700.035.7456844
TATGCCG48150.034.6692343
CCACGAG48350.034.66541320
CACGAGA48350.034.66541321
TCTCGTA48350.034.66541338
ACGAGAC48300.034.6547222
CGTATGC48300.034.6547241
TCGTATG48300.034.6547240
GTATGCC48250.034.64400542
TCTCCGA48250.034.64400511
CGAGACC48250.034.64400523
GACATCT48000.034.63696334
GGACATC47950.034.6261633
TGGACAT47950.034.6261632
TCCGAGC48150.034.62250513
CCCACGA48350.034.6188819
ATCTCCG48300.034.60814310
Produced by FastQC (version 0.11.2)