FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_46.35100000044906.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_46.35100000044906.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC107743.18364073777065RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT1546.174819566960705No Hit
ATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTTC240.9623095429029671RNA PCR Primer, Index 20 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCT190.7618283881315157No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTTGACATCTCGTATGCCGTC90.36086607858861264No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG90.36086607858861264No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG80.32076984763432237No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC60.24057738572574178No Hit
TATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTCTT60.24057738572574178RNA PCR Primer, Index 20 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTTTGGACATCTCGTATGCCGTC30.12028869286287089No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT30.12028869286287089No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTGGACCATCTCGTATGCCGT30.12028869286287089No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCTGTC30.12028869286287089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1150.043.0434845
ATGCCGT1300.038.07692744
AGCCCAC1400.036.96428317
ATCTCGT1400.036.96428337
ATCTCCG1400.036.96428310
CACATCT1400.036.9642837
GCCCACG1400.036.96428318
CCACGAG1400.036.96428320
CATCTCC1400.036.9642839
GAGCCCA1400.036.96428316
CACGAGA1400.036.96428321
CCCACGA1400.036.96428319
TACACAT1400.036.9642835
ACACATC1400.036.9642836
TCTCGTA1400.036.96428338
ACGAGAC1400.036.96428322
CGTATGC1400.036.96428341
CGAGCCC1400.036.96428315
CCGAGCC1400.036.96428314
TCGTATG1400.036.96428340
Produced by FastQC (version 0.11.2)