FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_45.351000000448eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_45.351000000448eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32133
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC9282.8879967634519033RNA PCR Primer, Index 20 (95% over 21bp)
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA1680.5228270002800859No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.5228270002800859No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.47303395263436343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.38589611925434913No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA1240.38589611925434913No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC1150.3578875299536302No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.323654809697196No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCC1000.31120654778576545No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.3018703513521924No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA910.28319795848504653No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATAC870.2707496965736159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.26452556561790064No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA740.2302928453614664No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGCTGTCTCTTATACACATCTCCG720.2240687144057511No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.2178445834500358No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA690.21473251797217813No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.19606012510503223No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG600.18672392867145926No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACA590.1836118631936016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.1836118631936016No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT590.1836118631936016No Hit
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.18049979771574395No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT580.18049979771574395No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA570.1773877322378863No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG560.17427566676002862No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA540.1680515358043133No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA530.16493947032645567No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA510.15871533937074037No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG490.15249120841502506No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC450.14004294650359445No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC450.14004294650359445No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG430.1338188155478791No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCCTGTCTCTTATA420.13070675007002147No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT410.1275946845921638No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.1275946845921638No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA400.12448261911430618No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC400.12448261911430618No Hit
GTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA380.11825848815859086No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA370.1151464226807332No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA360.11203435720287555No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCCTGTCTCTTATA360.11203435720287555No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCTC350.1089222917250179No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCCTGTCTCTTATACACATCTCC340.10581022624716024No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT340.10581022624716024No Hit
CTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA340.10581022624716024No Hit
GTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCTGTCT340.10581022624716024No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA330.10269816076930258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1100.045.045
ATGCCGT1200.041.24999644
GTATGCC1350.036.66666442
CCACGAG1350.036.66666420
TATGCCG1350.036.66666443
CACGAGA1350.036.66666421
TCTCGTA1350.036.66666438
ACGAGAC1350.036.66666422
CGTATGC1350.036.66666441
TGTGGAC1350.036.66666430
CGAGACC1350.036.66666423
CTCGTAT1350.036.66666439
ATCTCGT1300.036.34615337
CATCTCG1300.036.34615336
GACATCT1300.036.34615334
CCTGTGG1400.035.35714328
CCCACGA1400.035.35714319
ACCTGTG1400.035.35714327
TCGTATG1400.035.35714340
GGACATC1350.035.033
Produced by FastQC (version 0.11.2)