FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_44.351000000448c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_44.351000000448c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364327
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC191615.2592863004937875RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT42701.1720240333546512No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC12070.33129578647753255No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC10740.29479011986484666No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA9800.2689891224092642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA8560.23495376406360222No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG8250.22644492447718673No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA6220.17072574912098198No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC5470.1501398468957832No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA5340.14657162384341538No Hit
AAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAG4760.13065185945592833No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC4110.11281074419408937No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3910.10732117026736969No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA3690.10128263894797805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA207.024401E-445.00329630
TGCCGTC19550.044.19154445
GCCTAGG301.13790134E-437.50274321
AGGCCCA250.002104467536.00263625
ATAAACG250.002104467536.0026363
CCCGAAT250.002104467536.00263618
GCGGTAG250.00210588635.9976969
TAACCCG250.00210588635.99769615
GGCGGTA250.00210588635.9976968
ACCCGAA250.00210588635.99769617
ATGCCGT24650.035.04846644
CGTATGC25750.033.98811341
TCTCGTA25900.033.96500438
CATCTCG25850.033.9436736
GGACATC25850.033.68718333
CTCGTAT26000.033.6613139
ATCTCGT26250.033.5121437
TATGCCG25850.033.5084943
GTATGCC25950.033.46606442
TCGTATG26200.033.4043540
Produced by FastQC (version 0.11.2)