FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_43.3510000004489b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_43.3510000004489b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC54.716981132075472RNA PCR Primer, Index 20 (95% over 21bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT21.8867924528301887No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA21.8867924528301887No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGCGTTATCTCGTATGCCGTC10.9433962264150944No Hit
TTTTTTTTTTTTGGGGTACGATTTGATGTTACCTGATGCCTGTCTCTTATA10.9433962264150944No Hit
AAAGACTGGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGCTGTCTC10.9433962264150944No Hit
GCTAGGAACACTAATCCTGAAACTAGAAGGTACAGTGGCTGTCTCTTATAC10.9433962264150944No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCC10.9433962264150944No Hit
GTTATATATCAGACGAAAGTGAAGGAAAGGAACAAAACTGTGTTAGCTGGT10.9433962264150944No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTCTGTC10.9433962264150944No Hit
GTTTTGCTTCTACTGGATTCATCGTGTGGTTATTACACTTGCAGCTTTACC10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTAACATCTCGTATGCCGTC10.9433962264150944No Hit
TACTGATTCAGGCTCTGGGCTCCTCCCCGTTCGCTCGCCGCTACTGGGGGA10.9433962264150944No Hit
GTCTTCTTTATTCAAGAAGTTTGTAGCCATTTTCAGAATTCACTCAAGAAC10.9433962264150944No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATCTGTCTCTTATACACATCT10.9433962264150944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAAAA10.9433962264150944No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAAAGCCA10.9433962264150944RNA PCR Primer, Index 27 (96% over 28bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA10.9433962264150944No Hit
CATCTGAATGGCTTGTTTTTTAACTGAAACTGGTTGCAAACTGTATTGTCT10.9433962264150944No Hit
ACTGGAGATTTCTGTTCTCATTGAGTCCCCTTTAGGATACCCTGTCTCTTA10.9433962264150944No Hit
GTTTCTTAGGTATTCTTTTCCCCATCTTATCAACAAAGAAAAACATAGCTA10.9433962264150944No Hit
AGACTATCCTGTATGTGTGAGAGAATATTGCTGTCTCTTATACACATCTCC10.9433962264150944No Hit
GTTGAAAAGGATGTTGGTGCCCCTGGTTTCACTGACACTCGGCCGTAATGT10.9433962264150944No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACATTTCGACATCTCGTATGCCGTC10.9433962264150944No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAAA10.9433962264150944Illumina PCR Primer Index 8 (96% over 27bp)
GATGCCACTGCTTCCCGTACTCTGCGTTGATACCACCTGTCTCTTATACAC10.9433962264150944No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCAAATCTATC10.9433962264150944RNA PCR Primer, Index 27 (96% over 28bp)
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACGAAGTCT10.9433962264150944RNA PCR Primer, Index 27 (96% over 28bp)
ATACAGACATTTCTTAAAAGTTACCGTATCATTCACATGCCGTCTCTTATA10.9433962264150944No Hit
TCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGCTGT10.9433962264150944No Hit
TTTATAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC10.9433962264150944No Hit
GGCATATCAATGTGAACTTGAGAGACGTGCAATGTGAATAACGGACCACCC10.9433962264150944No Hit
TTTCAATATGATCCCATTTACTGTAGTCCAGTCTGGACATACTTAATCGCT10.9433962264150944No Hit
AGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGA10.9433962264150944No Hit
GTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAA10.9433962264150944No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGCTGACTC10.9433962264150944No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTA10.9433962264150944No Hit
CACAAACGCTTTATTTAAAGGAGCATCTCAGTCTGTCTCTTATACACATCT10.9433962264150944No Hit
AGTTCTCCATCACCTACAAGCCCGTAAAGCATGGCCGGCCCGGCATCGGGG10.9433962264150944No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG10.9433962264150944No Hit
ATTTTAGTGTCTTCCTTTGATGAATGCTCGAAAATGACCACACTACCTGTC10.9433962264150944No Hit
CATTTATATACTACTCGTGCGTAGATAATGACTGACATTGAAGACTTGAAT10.9433962264150944No Hit
GGATAACTACTGGAAACGGTAGCTAATACCGCATCTGTCTCTTATACACAT10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTTGACATCTCGTATGCCGTC10.9433962264150944No Hit
GTGGACCAAAACATAATTTGCTTGTAACTATTCTAGTCTAGGATCGTATGC10.9433962264150944No Hit
ATGATTAGCTTTGTCACTGTTTCACTACTCAGCATGGAAACAAGATGAACT10.9433962264150944No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC10.9433962264150944No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT10.9433962264150944No Hit
GAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCG10.9433962264150944No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAA10.9433962264150944RNA PCR Primer, Index 36 (96% over 29bp)
CTCTTATACACATCTCCGAGCCGACCAGTCCTTTGGACATCTCGTATGCCC10.9433962264150944No Hit
CGAATAAACCCAGCAAAAAGAGAACTGCATACTTAAATTTAGGATAGTCAT10.9433962264150944No Hit
CAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAAGGAGGTCAAGCCTC10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTTGGTCATCTCGTATGCCGTC10.9433962264150944No Hit
ATGTTACCCAACATGCTGTTGGCATTGTTGTAAACAAACAAGTTAAGGGCA10.9433962264150944No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGAT10.9433962264150944No Hit
AGTTTATTCTTAAATGTACAACAGCTCCAAGACAACACTTAATTCCCTGTC10.9433962264150944No Hit
CGATCAACTTGTTCTTCCTCTCCTCGTAAGTAAGTTCTGTCTCTTATACAC10.9433962264150944No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC10.9433962264150944No Hit
ATGCAGTTCGTGCCTGATTTAAGGTTTCAGTATCAGCTGTCTCTTATACAC10.9433962264150944No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACCTGTCTCTTATACACAT10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTTGGTCATCTCGTATGCCGTC10.9433962264150944No Hit
AGCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCTGTCTCT10.9433962264150944No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCTGTCTCTTATACA10.9433962264150944No Hit
GGGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTCTGTCTCTTATACAC10.9433962264150944No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCCTG10.9433962264150944No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCAC10.9433962264150944No Hit
TTTTTTTTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCTT10.9433962264150944No Hit
ACTGTAACTGATTAAATTCTGTGGCAATTCTCTCCAAAATTTGTCCAGTCA10.9433962264150944No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCTGTCTCTTATACA10.9433962264150944No Hit
CTGTTTTACAGCTCGACATCTCTCACTGTTCTCTTTGATTCGTCGTTCAAG10.9433962264150944No Hit
TGCTTGACGTGTTCCACTCTCACGTTGATTCTTTTCGCAAGAATTCGACCT10.9433962264150944No Hit
TATCTTCACAGGAAGTAAGTCACATCTGGAGAACTCTACGAGGAGTTGTAT10.9433962264150944No Hit
GGTGAAAGAAGCTGAGCTGAACACATTACGATGGATGATGGAAACATAAGA10.9433962264150944No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACTGTCTCTTATACACA10.9433962264150944No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACAAATACAC10.9433962264150944RNA PCR Primer, Index 27 (96% over 28bp)
GTTCGCCTCATTAACCTATGGATTCAGTTAATGATCTGTCTCTTATACACA10.9433962264150944No Hit
GGATCAAGGTCGGTGGCAAAGCTGGTGCACTTGGTGATTCTGTCACAATCA10.9433962264150944No Hit
ACATTGTGGTCCTACTACCACTGTTTTTGTCGGCAACATTTCCGAGAAAGC10.9433962264150944No Hit
GTCTTTCTCTGTTCATCACTTATGGAGCCACTAACAAATTCTCCATAACTC10.9433962264150944No Hit
TGATTAGAGATGTTGTTACTTACACTGAGCATGCGAGGAGGAAGACGGTGA10.9433962264150944No Hit
GGGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT10.9433962264150944No Hit
ACACACTATCATTAACTGAATCCATAGGTTAATGCTGTCTCTTATACACAT10.9433962264150944No Hit
CACTGAGGGTGTCGCTGAGAGCCTCTTCCTGCCCAGAACAGATTACAGCTT10.9433962264150944No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT10.9433962264150944No Hit
TTGATACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA10.9433962264150944No Hit
GCGTTGATACCACCCGTACTCCTGTCTCTTATACACATCTCCGAGCCCACG10.9433962264150944No Hit
AAGATGTATCGAAACTCACCCAAAACTATTGGATGCTGACTGTTTCCTCCA10.9433962264150944No Hit
GCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTA10.9433962264150944No Hit
GTACTGTCCTTGTATGATTCCTCACCCAATGTAACTAACCTGTCTCTTATA10.9433962264150944No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGTCGTCATCTCGTATGCCGTC10.9433962264150944No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTCTGTCTCTTATACACATC10.9433962264150944No Hit
GCACTGGTTCATCTTCCTTCTCAACTTACCTGTTGCCACTTGGAATATATA10.9433962264150944No Hit
ACACACACCAGCAGCACCCAGAAGAAGTTGAACATCATGAGAATGGTGCGA10.9433962264150944No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT10.9433962264150944No Hit
CTTTCCAATTGCTTTCTGCAAAGCCCGTTGTTTACTTGATTAACCTGAATG10.9433962264150944No Hit
GAAAGTTTCTCCAAAGTTAAAGCGACATCAGAAGACAAGAAGATCTGTCTC10.9433962264150944No Hit
GGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCTGTCTCTTATA10.9433962264150944No Hit
TAAGTCTCCTGTCCTTCATCCTCCAAAGTTTATACATTGCAGTACCTGTCT10.9433962264150944No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers