H23LNBCXX_l02n01_bir_0113_pb_41.35100000044858.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pb_41.35100000044858.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25506
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC	1356	5.316396142084216	RNA PCR Primer, Index 20 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGT	280	1.097780914294676	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCG	60	0.2352387673488591	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	52	0.20387359836901123	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	46	0.1803497216341253	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.1568258448992394	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.1529051987767584	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	38	0.14898455265427743	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	34	0.13330196816435347	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	33	0.1293813220418725	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	30	0.11761938367442955	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	29	0.11369873755194856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.1058574453069866	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	160	42.1875	45
CCTGTGG	175	38.571426	28
TATGCCG	175	38.571426	43
GACATCT	175	38.571426	34
CTGTGGA	175	38.571426	29
CGTATGC	175	38.571426	41
TGTGGAC	175	38.571426	30
AGACCTG	175	38.571426	25
CGAGACC	175	38.571426	23
GTGGACA	175	38.571426	31
CTCGTAT	175	38.571426	39
GAGACCT	175	38.571426	24
GTATGCC	180	37.5	42
ATCTCGT	180	37.5	37
CCACGAG	180	37.5	20
CATCTCG	180	37.5	36
CACGAGA	180	37.5	21
ACCTGTG	180	37.5	27
ACGAGAC	180	37.5	22
ATGCCGT	180	37.5	44