FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_39.35100000044814.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_39.35100000044814.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96890
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC2690627.76963566931572No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT57505.9345649705852No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG53925.565073795025286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC28200.043.95671545
TCTGCTT356.195094E-638.5661223
ATGCCGT35650.034.7708144
TGTTGAG300.005101877730.026872
AGTAGAG300.005101877730.026875
TATGCCG41300.030.01402743
TCTCGTA41450.030.01396238
CTCGTAT41400.029.9958739
ATCTCGT41550.029.94172537
CGTATGC41550.029.94172541
TCGTATG41600.029.90573940
GATCTCG41450.029.79686236
GTATGCC41650.029.76180842
TGATCTC41550.029.7251535
AGGATGT41700.029.67217429
ACGAGAC42100.029.65743622
GGATGTG41500.029.65254230
CGAGACA42200.029.6404723
CCACGAG42150.029.62225720
GAGACAG42250.029.60538924
Produced by FastQC (version 0.11.2)