FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_38.351000000447f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_38.351000000447f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46813
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC536311.456219426227756No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT24355.201546578941747No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG6131.3094653194625425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5650.044.20353745
ATGCCGT8550.029.21052644
TATGCCG9550.026.15183343
TGATCTC9600.026.01562735
GATCTCG9600.026.01562736
CTCGTAT9600.026.01562739
ATCTCGT9650.025.88082937
GTATGCC9700.025.74742342
TCTCGTA9700.025.74742338
CGTATGC9750.025.61538341
GGATGTG9700.025.51546530
TGTGATC9700.025.51546533
TCGTATG9800.025.48469440
GTGATCT9750.025.38461534
GATGTGA9850.025.12690231
ATGTGAT9850.025.12690232
CAGGATG9850.025.12690228
ACAGGAT9950.025.10050427
AGGATGT9900.025.029
GACAGGA10000.024.97500226
Produced by FastQC (version 0.11.2)