FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_37.351000000447ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_37.351000000447ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14101
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC1230.8722785618041273No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA280.19856747748386638No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC280.19856747748386638No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC230.1631089993617474No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1560173037373236No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA200.141833912488476No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCG170.1205588256152046No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT160.11346712999078079No Hit
GTGCATGGTAGGCAGTTTGACTGGGGCGGTCTCCTCCCAAAGTGTAACGGA160.11346712999078079No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11346712999078079No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG150.10637543436635699No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCA301.0636049E-437.6186263
ACGGGGG352.6887044E-432.1301916
GTACTTT903.0559022E-1027.5869921
ACTTTTT903.0559022E-1027.5869923
TACTTTT903.0559022E-1027.5869922
CTTTTTT1001.0422809E-924.828294
GTATCAA1356.002665E-1121.7352051
TACGGGC550.003681201320.519252
TATCAAC1854.0381565E-915.8608262
CAACGCA1854.0381565E-915.8608265
AACGCAG1854.2364263E-915.8045826
TACGGGG1003.8266543E-415.74379415
TCAACGC1905.7516445E-915.4434354
ATCAACG1905.7516445E-915.4434353
ACGCAGA1906.035407E-915.388677
CGCAGAG1958.511051E-914.994098
GCAGAGT2051.6480044E-814.262679
GAGTACG1901.0497024E-714.204926512
CAGAGTA2102.264278E-813.92308310
AGAGTAC2102.264278E-813.92308311
Produced by FastQC (version 0.11.2)