FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_35.3510000004479c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_35.3510000004479c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT66.896551724137931No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC33.4482758620689653No Hit
CTACTAAACAGAGTCTTTTTTGACTTGCCAAAAACACATCCTGTCTCTTAT11.1494252873563218No Hit
CATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCC11.1494252873563218No Hit
GGGTGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGACCCCTGG11.1494252873563218No Hit
CTCAACACTTGATCAAATAGACCTAACAGACATTTACAGAACACTCCACAC11.1494252873563218No Hit
GTCCAAGGGTCTACTGTCCCAACTGCAGAAACATCCGCTACTAAAGACTGT11.1494252873563218No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACATACACA11.1494252873563218Illumina PCR Primer Index 8 (96% over 27bp)
TATCCAAGGAGGAAGAGACCAAGAAATAAAACCTCCCACTTTGTCTGTACA11.1494252873563218No Hit
TATTAAGAGCGTGGACAAGAAGGACCCAACTGGAGCCAAGGTCACCACTGT11.1494252873563218No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCATATGATCTCGTATGCCGTC11.1494252873563218No Hit
GCACAGATCCTACCAACAGTTGATATTGTCTCAACAGGGTACCTGTCTCTT11.1494252873563218No Hit
AGCTCACAATATGTGCATTTTTTGTGTACGGGGCTGTCACTGTCTCTTATA11.1494252873563218No Hit
ATACACACACAAACCATTTTATTTTTTGGGCCATTACCCCATACCCCTTAT11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC11.1494252873563218No Hit
GTTCAGAAAGGTACTGGTACATAATAGTCTGTCTATCTGCTGTCTCTTATA11.1494252873563218No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCCTGTCTCT11.1494252873563218No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAGA11.1494252873563218RNA PCR Primer, Index 27 (96% over 28bp)
AACCCAGTGTGTTTCGACACACTATCATTAACGGAATCCATAGGTTAATGA11.1494252873563218No Hit
AGAGATAACCGCTGAAAGCATCTAAGCGGGAAACCCGTCTCAAGATAAGTC11.1494252873563218No Hit
AGTTTAGATAAAACACCAAAAAAGGGACTCAAAACATGAAGACTGTCTCTT11.1494252873563218No Hit
GCATAGTATTCCATGGTGTATACGTACCACATCCTGTCTCTTATACACATC11.1494252873563218No Hit
GAGTACGGGGGCTTGGTTCTTCATGAGCACATTTGATATAGCTCTTTTTCT11.1494252873563218No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAATTTACTGT11.1494252873563218Illumina PCR Primer Index 8 (96% over 27bp)
ACACTACCTGACTTCAAACTATACTACAAGGCTACCTGTCTCTTATACACA11.1494252873563218No Hit
CAATCAAATGGAATGAGAAATGAAGCCACAAAAGTATCTGCAATCAAAATA11.1494252873563218No Hit
GTTTTACTGTCATCTGGTTGTTTTCGGTTTGGATAAGCCATCGTCTCCCTG11.1494252873563218No Hit
GTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTT11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC11.1494252873563218No Hit
GATTATAGGCATGATGCTGTCCCCAGTCAGATTAATATTTTCAAGAGAAGC11.1494252873563218No Hit
TGTTAGGGGAGCAGGGGAGCATAAATGTAGAAAACATGATAAGCAACTGTC11.1494252873563218No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC11.1494252873563218No Hit
TCATATTGCGATATACAAACAATAAGCATGTTGTAGACCTGTCTCTTATAC11.1494252873563218No Hit
GTACGGGGACGGTTGTCCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAA11.1494252873563218No Hit
GTCAACTTGTTTATGAATGTTTTCCGTTACTTCTTATGCAACCAGTTATTG11.1494252873563218No Hit
TGTCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGCAGTACCT11.1494252873563218RNA PCR Primer, Index 27 (96% over 28bp)
CGTGAACACTGTCGTACTTGAACATGTAGGTCACTGTCTCTTATACACATC11.1494252873563218No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGGGGCAG11.1494252873563218No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAACACAA11.1494252873563218Illumina PCR Primer Index 8 (96% over 27bp)
TCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT11.1494252873563218No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACC11.1494252873563218No Hit
AAAAAGTACTCTGCGTTGATACCAGGTAAACTGTCTCTTATACACATCTCC11.1494252873563218No Hit
CTTATACACATCTCCGAGCCGAGACCCTAGAATATCTCGTATGCCGTCTTC11.1494252873563218RNA PCR Primer, Index 44 (96% over 25bp)
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAATAGACTAC11.1494252873563218RNA PCR Primer, Index 36 (96% over 29bp)
TCTCAGTGGTATCAACGCAGAGTACGGGCAGCTGTCTCTTATACACATCTC11.1494252873563218No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGCTGTCTCTTATA11.1494252873563218No Hit
GCACCCCATCATTTAATCTTCCGGCACCGGGCAGGCGTCACACCCTATACG11.1494252873563218No Hit
CATTTTAAGAGCCCAGTTTCCGTATTTGAGAGAAGATCCTTTCCCAATCTA11.1494252873563218No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCACGGAGTCA11.1494252873563218No Hit
CACCTATCTCACTCTAAAATCTCTCTCTGCCAATCTCATCTTCAACCTCTC11.1494252873563218No Hit
GCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCTGTCTCTTAT11.1494252873563218No Hit
ATCCATGTACAAACGTGAATCATACGGCAAAGAAGCAAATATTGGAGTGCG11.1494252873563218No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCCTGTCTCTTATA11.1494252873563218No Hit
GTCCGATTGGGGGGGGGGTCCCTTCAGAGACCCACGGGCCGGTGGCATCGG11.1494252873563218No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCC11.1494252873563218No Hit
GAATTCTAGTCAGACCAGTTGTCTGCTCTCGAACTCTGTTTCCTGTTAAGA11.1494252873563218No Hit
CTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGACTGTCTCTTATACA11.1494252873563218No Hit
GTCCTGGAGGGGGTTTATTCCATCACAGACACAGTCATCAACAACAGTCCA11.1494252873563218No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGTCGCTTATA11.1494252873563218Illumina PCR Primer Index 8 (96% over 27bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGCTGTCTC11.1494252873563218No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACATATGCCGAC11.1494252873563218Illumina PCR Primer Index 8 (96% over 27bp)
CCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG11.1494252873563218No Hit
GTTAAGATTTCCACATATCTTTTTGCAGAGCACACAATCTGTCTCTTATAC11.1494252873563218No Hit
ATATAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAACACC11.1494252873563218Illumina PCR Primer Index 8 (96% over 27bp)
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT11.1494252873563218No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTT11.1494252873563218No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCAGTCTCT11.1494252873563218No Hit
GGGATAACTACTGGAAACGGTAGCTAATACCTGTCTCTTATACACATCTCC11.1494252873563218No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGTTATCTCGTATGCCGTC11.1494252873563218No Hit
AGGTAACAATTACAGAGAATGATAAGCAAAATGCTAACACTGTCCCTTATA11.1494252873563218No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAC11.1494252873563218No Hit
GTCCTAATATGGTTTCAGACAACATTTTGAAGCTTGAACATAATCTGTCTC11.1494252873563218No Hit
CAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGCAGATA11.1494252873563218RNA PCR Primer, Index 36 (96% over 29bp)
GTACGGGATCCGGAAAATCAAGGCTGAGGCGTGATGACGCTGTCTCTTATA11.1494252873563218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1494252873563218No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCCTGTCTCTTAT11.1494252873563218No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA11.1494252873563218No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACTGTCTCTTATACA11.1494252873563218No Hit
TTCTTACGGAGATCAGGCTTACCTTTCTTAACAGTAGCCCTGTCTCTTATA11.1494252873563218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTT50.045.024
CTCTGCG50.045.09
GTTGATA50.045.015
TGCGTTG50.045.012
CTCCTGT50.045.037
ACCACTG50.045.021
GATACCA50.045.018
TGTCTCT50.045.041
CCTGTCT50.045.039
GTACTCT50.045.06
GTACTCC50.045.034
CTCTTAT50.045.044
ACTCCTG50.045.036
ACTCTGC50.045.08
CACTGCT50.045.023
TGATACC50.045.017
CTGCGTT50.045.011
CCACTGC50.045.022
TCTGCGT50.045.010
GTCTCTT50.045.042
Produced by FastQC (version 0.11.2)