H23LNBCXX_l02n01_bir_0113_pb_34.35100000044772.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23LNBCXX_l02n01_bir_0113_pb_34.35100000044772.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15861
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	377	2.3768993127797744	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT	87	0.5485152260261018	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACA	66	0.41611499905428406	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCT	56	0.3530672719248471	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT	51	0.3215434083601286	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCC	50	0.3152386356471849	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG	42	0.2648004539436353	No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACCTGTCTCTT	31	0.19544795410125468	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCT	26	0.16392409053653617	No Hit
CTATCATTAACTGAATCCATAGGTTAATGACTGTCTCTTATACACATCTCC	25	0.15761931782359245	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGACTGTCTCTTATAC	23	0.14500977239770507	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTC	23	0.14500977239770507	No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGACTGTCTCT	23	0.14500977239770507	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCA	22	0.13870499968476135	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGCTGTCTCTTATACACATCT	22	0.13870499968476135	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAA	22	0.13870499968476135	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACCTGTCTCTTA	21	0.13240022697181766	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACA	20	0.12609545425887397	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGT	19	0.11979068154593026	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGCTGTCTCTTATACACATCTCC	19	0.11979068154593026	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACCTGTCT	18	0.11348590883298658	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT	18	0.11348590883298658	No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA	17	0.10718113612004287	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTC	17	0.10718113612004287	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA	17	0.10718113612004287	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT	16	0.10087636340709917	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT	16	0.10087636340709917	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	40	6.1136234E-9	45.0	45
GGATGTG	50	4.3817636E-8	36.000004	30
GTATGCC	50	4.3817636E-8	36.000004	42
ATCTCGT	50	4.3817636E-8	36.000004	37
TGATCTC	50	4.3817636E-8	36.000004	35
TATGCCG	50	4.3817636E-8	36.000004	43
GATGTGA	50	4.3817636E-8	36.000004	31
TCTCGTA	50	4.3817636E-8	36.000004	38
GATCTCG	50	4.3817636E-8	36.000004	36
ATGCCGT	50	4.3817636E-8	36.000004	44
CGTATGC	50	4.3817636E-8	36.000004	41
GTGATCT	50	4.3817636E-8	36.000004	34
TCGTATG	50	4.3817636E-8	36.000004	40
TGTGATC	50	4.3817636E-8	36.000004	33
GCCCACG	55	1.0133226E-7	32.727276	18
AGGATGT	55	1.0133226E-7	32.727276	29
CACGAGA	55	1.0133226E-7	32.727276	21
CCCACGA	55	1.0133226E-7	32.727276	19
ATGTGAT	55	1.0133226E-7	32.727276	32
ACGAGAC	55	1.0133226E-7	32.727276	22

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content