FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_33.35100000044759.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_33.35100000044759.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15681
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC12297.837510362859512No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGT4682.984503539315095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCG1470.937440214272049No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA220.14029717492506857No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC220.14029717492506857No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC180.11478859766596518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1250.045.00000445
ATGCCGT1950.028.84615344
GTGATCT2150.028.25581434
GGATGTG2100.027.85714330
ATCTCGT2100.027.85714337
GACAGGA2100.027.85714326
CCACGAG2100.027.85714320
CACGAGA2100.027.85714321
TCTCGTA2100.027.85714338
GATCTCG2100.027.85714336
CGTATGC2100.027.85714341
TCGTATG2100.027.85714340
CGAGACA2100.027.85714323
CAGGATG2100.027.85714328
CTCGTAT2100.027.85714339
TGTGATC2100.027.85714333
TATGCCG2050.027.43902443
AGGATGT2150.027.20930329
TGATCTC2150.027.20930335
GATGTGA2150.027.20930331