FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_32.3510000004474c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_32.3510000004474c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177567
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC2761015.54906035468302No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT44862.5263703278199214No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG8220.4629238540945108No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT5660.3187529214324734No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC2130.11995472131646084Illumina PCR Primer Index 3 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC28950.042.89940645
ATGCCGT33500.037.1399344
CCACGAG34550.036.271720
AGCCCAC34500.036.25905617
CCCACGA34600.036.21928419
ACGAGAC34550.036.2065822
CACGAGA34650.036.16702321
GAGCCCA34600.036.1542616
CGTATGC34500.036.12862841
GCCCACG34700.036.11490618
GTATGCC34450.036.0504442
CGAGACC34650.036.0371623
TCTCGTA34550.036.01122338
AGACCAT34550.036.01122325
CATCTCG34500.035.998236
TCCCCTG250.002102840235.998245
CGAGCCC34750.035.99819615
ATCTCGT34600.035.95918337
CTCGTAT34600.035.95918339
TATGCCG34550.035.94610243
Produced by FastQC (version 0.11.2)