FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_31.35100000044722.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_31.35100000044722.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170677
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC9572556.085471387474584No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT2002511.732688059902623No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG40402.36704418287174No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT6940.40661600567153156No Hit
ATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCTTC2090.12245352332183011Illumina PCR Primer Index 3 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC100000.043.98692745
ATCCGAC301.1359404E-437.4995130
CCATCCG301.1359404E-437.4995128
ATGCCGT120500.036.48500444
CGTCTTC250.002102229535.9995345
TCCGAGC126050.035.25340713
CCGAGCC126200.035.24716614
AGCCCAC125900.035.22392717
GAGCCCA126050.035.2177116
ATCTCGT125800.035.21615237
GCCCACG125950.035.2099418
CGAGCCC126350.035.2053215
CCCACGA125850.035.20216419
TCTCGTA125850.035.18428838
CACGAGA125800.035.1803821
CTCCGAG126450.035.1774812
CATCTCG125700.035.1725736
ACGAGAC125700.035.1725722
CCACGAG125900.035.1703120
TCTCCGA126500.035.1635711
Produced by FastQC (version 0.11.2)