FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_30.351000000446fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_30.351000000446fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192448
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC6427433.39811273694712No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT2296911.935172098436981No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG25831.3421807449285001No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT9200.47805121383438637No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACA207.018834E-444.9997431
CGACATC253.8787988E-544.9997433
CCATCCG253.8787988E-544.9997428
CCGACAT207.018834E-444.9997432
TGCCGTC68100.043.2156145
ATCCGAC250.002102820235.99979430
TAACAAT250.002102820235.9997944
CATCCGA250.002102820235.99979429
GCCGTCT2700.034.99979845
ATGCCGT92700.031.74738744
CCCACGA96600.030.79174619
CACGAGA96450.030.76965121
CCACGAG96450.030.76965120
GCCCACG96550.030.76108718
ACGAGAC96400.030.7389322
AGCCCAC96800.030.7281317
CGTATGC96300.030.72412141
TATGCCG95800.030.72007443
GAGCCCA96800.030.70488516
CCGAGCC96950.030.70379414
Produced by FastQC (version 0.11.2)