FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_3.35100000044390.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_3.35100000044390.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102412
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC2996629.260242940280435No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGT48754.760184353396086RNA PCR Primer, Index 44 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCG9320.9100496035620825No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT3750.36616802718431435RNA PCR Primer, Index 44 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCTTC1920.18747802991836895RNA PCR Primer, Index 37 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCT206.9867185E-445.0214921
GGAATCG207.003565E-444.999514
TGCCGTC30400.043.96333745
ATGCCGT35550.037.5945344
CGTCTTC301.1327485E-437.4995945
TCCGAGC36950.036.59635513
CTCCGAG37000.036.546912
CCGAGCC37000.036.546914
CGTATGC36950.036.53546541
TCGTATG36950.036.53546540
TATGCCG36650.036.5275743
GTATGCC36750.036.489442
CTCGTAT37000.036.4860939
GAGCCCA37000.036.4860916
TATCTCG36850.036.45143536
CGAGCCC37100.036.44839515
GCCCACG37050.036.4368518
AGCCCAC37050.036.4368517
CACGAGA36950.036.4136821
TCTCGTA36950.036.4136838
Produced by FastQC (version 0.11.2)