FastQCFastQC Report
Fri 12 Feb 2016
H23LNBCXX_l02n01_bir_0113_pb_28.351000000446c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23LNBCXX_l02n01_bir_0113_pb_28.351000000446c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461879
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTC7634616.529437363465323No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT328837.119397071527392No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCG31930.6913065976153928No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC27270.5904143725954201No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA22040.47718125309875525No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTA21220.45942768560597036No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC18930.4098476007785589No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA18280.39577465093671715No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT17560.3801861526503694No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA16000.3464110730299494No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTT12830.27777837918589066No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACTGTCTCT12570.272149199249154No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA11740.2541791248357254No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTC11240.24335377880353948No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCT11150.24140521651774596No Hit
TTATACACATCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGTCT10460.2264662389933294No Hit
GTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA9530.2063310953734636No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC9320.20178445003994552No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC9320.20178445003994552No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCCTGTCTCTTATACACATCTC9120.19745431162707114No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACTGTCTCTTATA8570.18554643099166665No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAGTACTTTTTCTGTCTC8310.17991725105492998No Hit
GAGTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTC8110.17558711264205562No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACCTGTCTCTTA7820.16930841194338778No Hit
AAAAAGTACTCTGCGTTGATACCCGTACTCTGCGTTGATACTGTCTCTTAT7090.15350340673639634No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC7040.15242087213317773No Hit
TATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTATA6970.1509053236886717No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG6950.15047230984738427No Hit
GGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGAGC6400.13856442921197976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTCTGTCTCTTATACACATCTCCGA6080.1316362077513808No Hit
AAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTTAT6000.12990415238623101No Hit
TATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT5710.1236254516875632No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5580.12081086171919486No Hit
GGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCACG5290.11453216102052702No Hit
AAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCC5280.1143156540998833No Hit
AAAAAGTACTCTGCGTTGATACCACCCGTACTCCTGTCTCTTATACACATC5160.11171757105215868No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACCTGTCTCTTATACACATCTCCG5160.11171757105215868No Hit
GTATCAACGCAGAGTACGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA5060.10955250184572149No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAGAGTACTTTCTGTCTCTTAT5040.10911948800443405No Hit
GTACGGGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCG4630.10024270425804162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC83700.044.24656745
CGGGCTG301.13897775E-437.4993717
TATCGAT301.13897775E-437.499377
GCCGTCT1400.035.3565545
CGGTTCG352.8180605E-432.1423222
ACGGGCT352.8180605E-432.1423216
CCCCCGG352.8180605E-432.1423218
CCGGTTC352.8180605E-432.1423221
GGTTCGC352.8180605E-432.1423223
ATGCCGT116750.031.70182244
TATGCCG120550.030.72117443
CGTATGC121000.030.69989641
CATCTCG121200.030.63067236
TCGTATG121200.030.63067240
GTATGCC121000.030.58832742
ATCTCGT121600.030.56691737
GACATCT121150.030.55045734
TCTCGTA121600.030.54841638
CTCGTAT121400.030.54314239
ACGAGAC122100.030.51545722
Produced by FastQC (version 0.11.2)